高剂量电离辐射对松果腺细胞照射后影响.docVIP

高剂量电离辐射对松果腺细胞照射后影响 　　 【摘要】 目的 主要研究高剂量电离辐射对松果腺细胞照射后不同时间点的影响。方法 采用流式细胞术检测松果腺细胞凋亡。采用放免分析法，检测松果腺细胞中cAMP含量。采用高效液相色谱分析法，检测松果腺细胞中MLT 含量的变化。结果 ①小鼠受6 GyX射线全身照射后8、48、120、168 h，松果腺细胞凋亡率增加，48 h达到最高。②小鼠受6 GyX射线全身照射后8、48、120、168 h，松果腺细胞中cAMP含量均降低，48 h达到最低。③小鼠受6 GyX射线全身照射后8、48、120、168 h，松果腺细胞合成和分泌MLT 功能减弱，48 h达到最低。结论 高剂量电离辐射对松果腺细胞功能有抑制作用，48 h达到最低。? 　　【关键词】 　　松果腺；电离辐射；褪黑素；细胞凋亡 　　 　　Effect after ionizing irradiation on function of pineal gland cells 　　 　　WANG Feng, AI Yiqin, LI Rong?qing. 　　The First Affiliated Hospital of Kunming Medical College,Kunming 650032, China 　　? 　　 　　【Abstract】 Objective 　　To research the effects different points of time after ionizing irradiation on function of mouse pinealocytes. Methods The contents of cAMP in mouse pinealocytes were measured with radioimmunoassay. The result MLT pinealocytes measured with high performance liquid chromatography.The percentage of pinealocytes apoptosis in mice was determined with flow cytometry. Results The percentage of pinealocyte apoptosis was observed time point to 8,48,120,168 hours after irradiation with 6 Gy in mice,and was highest 48 hours after irradiation.The content of cAMP in mice pinealocyte reduced time point to 8，48，120，168 hours after irradiation with 6 Gy, and was lowest 48 hours after irradiation. The content of MLT secreted by mouse pinealocytes decreased time point to 8，48，120，168 hours after by irradiation with 6.0 Gy X?rays, and was lowest 48 hours after irradiation. Conclusion High dose ionizing radiation has the inhibiting effect to the fuction of pinealocytes, and was lowest 48 hours after irradiation.? 　　【Key words】 　　Pinealgland; Melatonin; Ionizing radiation; Apoptosis 　　? 　　 　　? 　　作者单位：650032昆明医学院第一附属医院放疗科 　　 　　 　　 　　近年来随着松果腺研究的不断深入，松果腺对机体重要性越来越受到人们重视，而有关高剂量电离辐射对松果腺功能影响目前报道资料很少。我室以往研究表明，低剂量电离辐射可激活松果腺细胞功能，使松果腺分泌褪黑素(melatonin, MLT)能力增加[1]。本实验在此基础上研究高剂量电离辐射对松果腺细胞照射后不同时间点的影响，为肿瘤头部放疗对松果腺造成的影响提供一个实验依据。? 　　1 材料和方法? 　　1.1 主要试剂与仪器? 　　1.1.1 试剂 cAMP检测试剂盒(中国科学院原子能研究所)， 褪黑素(Sigma