过氧化氢对兔心肌的浆网膜流动性及钙—转移腺苷三磷酸酶活性的影响.doc

过氧化氢对兔心肌浆网膜流动性及钙—转移腺苷三磷酸酶活性的影响 BIBLIDltSSN0253—9756中国薛理批PI事4∞如妇3i~ica]993amp;p}14(5)l393--396 EffectsofhydrogenperoxideonmembranefluidityandCa(一trans— portingATPaseactivityofrabbitmyocardialsarcoplasmicreticulum suZhi,YANXiangDong.,LIYuan-Jian,CHENXiu (DepartmentofPharmacology,Hu-nanMedicalUniversity,Changsha410078,China) ^磷订nIACTThisstudywastoinvestigatetheeffects ofhydrogenperoxideonmembranefluidityandCa一 ATPaaeactivityofrabbitmyocardial$1Rrcoplasmic retlcolIlIn(sR).ThemembranefluidityofSR机s monitoredbymeasuringthechangesinthesteadystate fluorescenceanisotropies(^)usingdiphenhexamene asaprobe.TheCa外_ATP啪activitywasdeter- minedbyassayingtheamountofinorganicphosphate (Pigt;releasedfromATP.Itwasfoundthatthemem- brahefldidity(:0.154土0.01450.113土0.010,P lt;0.01)andCa抖一ATPaseactivity(3.1土1.325.3 土z.4/~molP?h-i/mgprotein,=Plt;0.01)werere- ducedinSRexposedtoH:Oi(2mmo1.L)for40 m_m.m20ng?III1completelypreventedthe SRdamagescausedbyH2Oz.H:~eopardlzedtheSR inaconcentration--andtime--dependentmsrxneraslea-- suredbychangesinyavaluesandCa一ATPaseactivi- ties.whichwenegativelycorrelated(rffiO.981,Plt; 0.01).TheseresultssuggestthatH2O:producesdys— functionsoftherabbitmyoeardiaISR.andthattheal— teratlonofmembraneflu/ditymaybeoneofthemecha— msmsresponsibleforthedecreaseofCa0+一ATPase activity. KEYWORDSmyocardinmfsarcoplasmicreficulum; hydrogenperoxide;membranefluidityICa.-trans— portingATPase Hydrogenperoxideisawellknownoxi— dantandphysstkeyroleinmyocardial ischemiaandreperfusioninjury.一.The processesthathavebeenproposedasfactors inperoxide—inducedcellinjuryincludedecrease |nATPconcentration,disturbancesofintra— cellularCa抖homeostasis.andanincreasein Received1992?11.12Accepted1993-04-24 SupportedhyiiGrantforYoungScie~dstsfromtheMJn- trvofPublicl-]ealthoChi NowmtResearchSevtiaaPAarmacology,Hu-,umMedl- cUniversi~y,Clumgsha41∞,略 Towhomcor~pondenceshouldbe皇ddressed. oxidi~x*dsulfhydrils.H2Ozcouldreducethe membranefluidityoferythrocytesandcar— diomyocytesC53.Butthedetailedmechanisms ofcell