uv-b胁迫下黄花蒿基因表达差异及青蒿素生物合成的研究-difference of artemisia annua gene expression and artemisinin biosynthesis under uv - b stress.docx

中文摘要 UV-B 胁迫下黄花蒿基因表达差异及青蒿素生物合成的研究 过氧化物酶活力显著提高。通过 ELISA 方法测定了黄花蒿叶片中内源植物激素脱落 酸的含量，发现在处理第 8 天和第 10 天显著高于对照组，分别达到对照的 2 倍和 1.5 倍。综上所述，本课题首次从转录组水平全面研究了药用植物黄花蒿对 UV-B 胁迫的 响应。研究发现使用低剂量的 UV-B 照射（1.44 kJ m-2 d-1）处理黄花蒿盆栽苗可以提 高青蒿素的含量。我们分析筛选出与黄花蒿 UV-B 胁迫响应相关的基因，并研究了活 性氧等信号物质在 UV-B 胁迫下的动态变化。本研究为有效利用 UV-B 刺激青蒿素合 成以及研究黄花蒿 UV-B 胁迫响应机制提供了参考和依据。关键词：中波紫外线；黄花蒿；青蒿素；微阵列芯片；基因表达作者：潘魏松 指导教师：王剑文UV-B 胁迫下黄花蒿基因表达差异及青蒿素生物合成的研究英文摘要The transcriptome responses involved in artemisinin biosynthesis in Artemisia annua L.under UV-B radiation AbstractArtemisinin, an endoperoxide sesquiterpene lactone produced by the traditional Chinese medicinal herb Artemisia annua L., is an effective drug against cerebral malaria and chloroquine-resistant strains of Plasmodium falciparum. There is an enormous demand for artemisinin supply all over the world. However, its low content in A. annua limits the commercialization of artemisinin production greatly. Attempts were made to improve the artemisinin production by optimizing chemical and physical environmental factors or by elicitation of abiotic and biotic factors. Ultraviolet B (UV-B) radiation (wavelength range 280 to 320 nm), a natural component of solar radiation, has been proved to affect humans, animals, plants and ecosystem. Moreover, after UV-B irradiation, plants display diverse morphological and physiological responses likely to be involved in signal transduction cascades and gene expression in the biosynthesis of secondary metabolites.In the present study, to study the effect of UV-B on gene expression and artemisinin biosynthesis, a low dose (1.44 kJ m-2 d-1) of UV-B radiation was applied to A. annua seedlings. The mechanism of induction has also been investigated preliminarily here.Firstly, to study the physiological responses in A. annua, about 2-month-old plants were treated with UV-B for 10 days. 10-day period UV-B treatment (1 h per day) enhanced the content of artemisinin and photosynthetic pigments in A. annua leaves, also caused foliar injury and