沙棘多糖的提取纯化及体内、体外功能研究-生物化学与分子生物学专业论文.docxVIP

目录1引言..........................................................................11.1多糖的介绍 ...................................................... 11.2植物多糖的研究进展 .............................................. 11.2.1增加巨噬细胞的生物学功能 ...................................... 11.2.2激活 T 和 B 淋巴细胞11.2.3调节多种炎性因子的分泌 ........................................ 21.2.4补体的激活作用 ................................................ 21.2.5抗肿瘤作用 .................................................... 21.2.6降血糖、降血脂的作用 .......................................... 21.3沙棘的研究进展 .................................................. 21.3.1沙棘概况 ...................................................... 21.3.2沙棘多糖的介绍 ................................................ 沙棘多糖的提取和纯化 ........................................ 沙棘多糖的主要生物学功能 .................................... 41.4研究的目的及意义 ................................................ 42沙棘多糖的提取纯化 ............................................................52.1材料 ............................................................ 52.1.1实验材料及试剂 ................................................ 52.1.2仪器 .......................................................... 52.2实验方法 ........................................................ 62.2.1沙棘多糖的提取 ................................................ 预处理过程 .................................................. 回流脱脂 .................................................... 超声裂解 .................................................... 热水提取 .................................................... 浓缩 ........................................................ 脱蛋白和蛋白质含量的测定 .................................... 醇沉 ........................................................ 抽滤 ........................................................ 72.2.2多糖的纯化 .................................................... 脱色 ........................................................ 透析 ........................................................ 分级沉淀 .................................................... 7多糖的 Sephad