青岛地区生物气溶胶中微生物活性分析-环境科学专业论文.pdf.docxVIP

究，得出青岛地区生物气溶胶中微生物活性平均水平为春季最高，秋季次之，冬季最低；且春季生物气溶胶中微生物活性水平的波动较大，而秋季和冬季生 物气溶胶中微生物活性水平较为稳定。生物气溶胶中微生物活性水平的月变化 范围为 0.41-4.85ng/mL 荧光素钠溶液，平均水平为 0.58-2.51ng/mL 荧光素钠溶 液。其中，2012 年 5 月微生物活性平均水平较高；2012 年 10 月微生物活性平 均水平次之；2013 年 1 月微生物活性平均水平最低。雾霾天气会导致微生物活 性水平的升高。青岛地区生物气溶胶中微生物活性与大气温度呈显著性正相关（P0.01）, 当相对湿度在 31%~89%的范围内时，微生物活性与大气相对湿度呈显著负相关（P0.05）。通过多元线性回归模型分析，得出生物气溶胶中微生物活性水平38.5%的变化与大气温度和大气相对湿度（31%-89%）相关。 通过对青岛地区气溶胶粒子数浓度与生物气溶胶中微生物活性的相关性分析，得出生物气溶胶中微生物活性与大于 2μm 粒级的粒子数浓度呈显著性相关（P0.01）；与 1-2μm 粒级的粒子数浓度也呈现显著性相关（P0.05）；而与0.7-1μm 粒级的粒子数浓度无显著性相关。春季生物气溶胶中微生物活性与2μm 粒级的粒子数浓度都有明显的相关性（P0.05）；而秋、冬季生物气溶胶 中微生物活性仅与5μm 粒级的粒子数浓度有显著的相关性（P0.05）。此外， 青岛地区生物气溶胶中微生物活性与 PM2.5 质量浓度无显著相关性。关键词：生物气溶胶；微生物；微生物活性； FDA 水解法Microbial activity in bioaerosols in Qingdao areaAbstractBioaerosols are airborne particles or large molecules that are alive, carry living organisms or are released from living organisms, such as bacteria, fungi, virus, pollen, cell debris, and biofilms. Airborne microorganisms can affect global climate change, environmental quality and human health. Microbial activity is a state or property of physiological process or active in microorganisms. Compared to the total amount and concentration of microbes in bioaerosols, microbial activity is more important to understand the impact of bioarosols on the environmental quality and human health.Based on the analysis method of microbial activity in soil, we developed the measurement method of total microbial activity using FDA （Fluorescein diacetate）hydrolysis in bioaerosols. Bioaerosols samples were collected on polycarbonate membranes, using a six-stage culturable microorganism FA-1 cascade impactors, with a flow rate of 28.3 L·min-1 for 40 min. 150μL 40% polyethylene glycol solution was used to coat on each polycarbonate membrane before collection. After sampling, bioaerosols samples collected on polycarbonate membranes were cut up and put into 100mL conical flask with 20mL physiological saline. The flask was then shaken at37℃ for 40min with 150r/min. 1