沙地雀麦质膜水孔蛋白基因(bipip)和actin基因克隆与序列分析-生物化学与分子生物学专业论文.docxVIP

Cloning and Sequence Analysis of Plasma Membrane Instrinsic Protein Gene (BiPIP) and Actin in Bromus ircutensis Kom.AbstractPlant aquaporin formed water selective passageway that mediate the water-transme- mbrane transportation of inside the cell or intercellular and adjust water balance in plants, which may play an important role to improve the water physiology integration of clonal plants and the salt, drought resistance. Study to Bromus ircutensis Kom. for material, Full-length cDNA sequence of BiPIP1-1 and BiAct respectively ware acquired by RT-PCR and RACE technology. Their structure of sequence of nucleotide and amino acid were analyzed by bioinformatics software. The mainly results as follows:BiPIP1-1 full-length cDNA sequence consists of 1077bp, which includes the length of 54bp of the 5′ untranslated region, followed by a 156bp of 3′ untranslated region, and finally 867bp of the open reading frame encoding 288 amino acids. Amino acid sequence of BiPIP1-1 not only have the MIP family of signal sequence, but also the characteristics of plasma membrane water channel protein signal sequence. Phylogenetic tree constructed showd that BiPIP1-1 are PIP1 subfamily members, which are very closer in relationship with HvPIP1-1. Tertiary structure of BiPIP1-1 contains six transmembranestructure (Ⅰ ~ Ⅵ, connected by five loops) and carboxyl terminusa and amino terminus.The two NPA boxes (Asn-Pro-Ala) is located in the center of the spiral, forming a two-way transportation through the water. Cloning of plasma membrane instrinsic protein gene in Bromus ircutensis Kom. is helpful for researching aquaporin expression of the molecular level in roots and the influence mechanism of water in the process of physiology integration.BiAct full-length cDNA sequence consists of 1310bp, which includes the length of 44bp of the 5′ untranslated region, followed by a 135bp of 3′ untranslated region, and finally 1131bp of the open reading frame encoding 376 amino acids. Amino acid sequence