不同浓度葡萄糖 胰岛素对兔视网膜müller细胞糖原合成代谢影响-effect of different concentrations of insulin glucose on glycogen synthesis and metabolism in rabbit retinal m ü ller cells.docxVIP

(2)High glucose could stimulate Müller cells growth and GLUT-1, GS expression,strengthened Müller cells transporting glucose, synthesizing and storeing glycogen. But along with culture time extension, high glucose arrested Müller cells growth and caused Müller cells more diacaustic, some cells neurite retracted. The OD value wasless and less along with culture time extension and glucose concentration increasing. GLUT-1 expression increased but GS expression dropped after 3 days. Cells in high glucose contented more glycogen than normal group, cells in high glucose for 3days or 5days also contented more glycogen than for 1day. Part of cells in the group with 50mmol/L glucose had floated , and same of cells underneath were also fragmentized, these groups OD value were low correspondingly, and cells contented too muchglycogen , GLUT-1, GS expression decreased obviously.（3）In the 1 day group,insulin could stimulate the rabbit retinal Müller cell to up-regulate the expression of GLUT-1 and GS, and result in the significant increase intracytoplasm glycogen, especially the concentration of insulin is 4U/ml. After 3d , the insulin cuold inhibitgrowth of Müller cell : the increase of cell diffraction, the retraction of neurite, andmany floating cells in higher concentration of insulin. The expression of GLUT-1 and GS is up-regulated in 1, 4U/ml insulin, but down-modulated in 8, 12U/ml insulin. For5 days, the effect of insulin on retinal Müller cells is obvious : many deactivativecells in every high concentration of insulin group, however, the expression of GLUT-1 and GS still is more than normal group , but less than that of 3d. The most significant increase of intracytoplasm glycogen still are these grupes of 1, 4U/ml insulin.Conclusion: (1)Retinal Müller cells can be cultured by the explant tissue culturemethod, the purity of Müller cells can reach to more than 95%; (2)High glucose significantly stimulate the growth of Müller cells at early stage, upregulate the expressi