布地奈德对鼻息肉上皮细胞gr-a表达的影响-effect of budesonide on gr - a expression in nasal polyps epithelial cells.docxVIP

incubatedwithbudesonidefrom1×10-7Mto1×10-6M,nasalepithelialcellsweregraduallyshrunkenastimegoingby.Theaveragesurvivalratesofnasalpolypsepithelialcellswererespectively(96.573±3.920)%and(96.559±3.101)%in1×10-8Mgroupand5×10-8Mgroup.Neitherofthemwerelowerthanthoseinthecontrolgroup(100%,P﹥0.05).Therewasnodifferenceintheaveragesurvivalratesofnasalpolypsepithelialcellsbetween1×10-8Mgroupand5×10-8Mgroup(P﹥0.05).Theaveragesurvivalrateofnasalpolypsepithelialcellswasthelowestin1×10-6Mgroup(29.284±0.311)%comparingwiththatin1×10-7Mgroup(89.215±1.198)%,(P0.05)and5×10-7Mgroup(52.833±0.125)%,(P0.05).Theaveragesurvivalrateofnasalpolypsepithelialcellsin1×10-7Mgroupwassignificantlylowerthanthatinthecontrolgroup(P0.05),whilehighterthanthatin5×10-7Mgroup(P0.05).(3)TheaverageexpressionlevelofGR-αmRNAin6hgroup(0.778±0.053)wassignificantlylowerthanthatinthe0hgroup(1.196±0.090,P0.05),whilehighterthanthatin12hgroup(0.559±0.024,P0.05).Comparedwith0hgroup,thereweresignificantlylowerexpressionlevelsofGR-αmRNAatboth24h(0.530±0.025)and48h(0.510±0.009)(P0.001);ExpressionofGR-αmRNAateither48hor12hwasnotsignificantlydifferentfromthatat24h.Conclusion:(1)ImmunohistochemicalsrudyshowedthatGR-αexpressioninnasalpolypsaremainlyexpressedinthenasalepithelium,glands,endothelialcellsandinflammatorycells,andmainlyexpressedinthecytoplasm.(2)Budesonideof1×10-7M～1×10-6Mcaninhibittheproliferationofnasalpolypsepithelialcellsandreducetheiraveragesurvivalrate.Budesonideof0～5×10-8Mhaslittleinfluenceontheaveragesurvivalrateofnasalpolypsepithelialcells.(3)Budesonideof1×10-8McansignificantlydownregulateexpressionsofGR-αmRNAinthenasalpolypsepithelialcellsat6hand12h.At1×10-8M,budesonidecausedatime-relatedexpressionsdownregulationofGR-αmRNAwhosemaximaleffectwasreachedat12h.Keywords：Glucocorticoid；GlucocorticoidReceptor；NasalPolyps；EpithelialCells；缩略词表GCglucocorticoid糖皮质激素GRglucocorticoidreceptor糖皮质激素受体RTreversetranscription逆转录PCRpolymerasechainreaction聚合酶链反应PBSphosptatbufferedsaline磷酸盐缓冲液mRNAmessengerribonucleicacid信使核糖核酸DA