不同配伍中药对湿热证ⅰ型变态反应大鼠模型的疗效对比研究-comparative study on the curative effect of different compatibility of traditional chinese medicine on type ⅰ allergic rat model of damp-heat syndrome.docxVIP

中文摘要目的：探讨三物黄芩汤及其加味对湿热证Ⅰ型变态反应SD大鼠的影响。方法：选用SD大鼠56只，雌雄各半，随机分为七组，即空白组、模型组、三物黄芩组、三物黄芩加当归组、三物黄芩加白术组、三物黄芩加当归白术组和阳性对照组（咪唑斯汀组），除空白组外，其余各组以高脂高糖辛辣饮食和高温高湿环境加I型变态反应的综合方法建立湿热证I变态反应动物模型。治疗组采用灌胃给药，治疗7天后，处死所有大鼠，采用ELISA法检测大鼠血清中总IgE含量；HE染色，普通光镜下观察大鼠胸腺、脾脏、肝脏、胃、十二指肠、小肠、结肠、直肠和皮肤等组织病理变化。结果：1.不同中药组方均能降低模型大鼠血清总IgE水平，与模型组相比有极显著差异（P0.01），而中药组间差异无显著性。2.不同中药组方均能减轻模型大鼠胃肠道血管充血和炎性细胞浸润，并能减轻肝脂肪变，胸腺和脾脏（HE染色）、皮肤未发现病理改变。结论：中药各组方能有效对抗I型变态反应，能减轻胃肠道炎症反应，并有保护肝脏的作用；而中药各组方间的疗效差异无显著性。关键词：不同中药组方湿热证Ⅰ变态反应疗效对比AbstractObjective：Toexploretheeffectof“Sanwuhuangqintang”anditsplusminusonthetypeⅠallergicSDratswithDampness-Heat.Methods：56ratsweredividedintosevengroupsrandomly：theblankgroup,themodelgroup,the“Sanwuhuangqintang”plusangelicaegroup,“Sanwuhuangqintang”plusrhizomegroup,the“Sanwuhuangqintang”plusrhizomeandangelicaegroup,thepositivecontrolgroup(Mizolastine).Excludingtheblankgroup,theallergicanimalmodelwithDampness-Heatwasestablishedinthemoistheatcircumstancebythecombinedmethod,includingtartfoodwithaffluentfatandsugarplustypeⅠallergy.Alltheratsofthetreatedgroupswereirrigatewithdrug,after7days,alltheratswereexecuted,AdoptingELISAtodetectthecontentofserumaltotalIgEoftherats,themicrostructureofmajororgansuchasthymusspleenliverstomachandsoonwasobservedunderopticalmicroscopewithimageanalysissystem.Results：1.AlltheherbalprescriptioncouldcutdownthecontentofserumaltotalIgE,comparingwiththemodelgroup,therewassignificantdifference(P＜0.01),butnoobviousdifferencebetweenalltheherbalgroups.2.Alltheherbalprescriptioncouldeasegastrointestinalbloodvesselextensionandinflammatorycellinfiltrate,easefattychangeoftheliver,butnochanginthymus、spleenandskin.Conclusions：AlltheherbalprescriptioncanresisttypeⅠallergicreactioneffectively,easegastrointestinalInflammatoryreactionandprotectliveroftherats。Buttherearenonotabledifferencebetweenalltheherbalgroups.Keywords：Differentherbalprescription;Dampness-HeattypeⅠallergicreaction;effectcomparement.2声明硕士学位论文不同配伍中药对湿热证Ⅰ型变态反应大鼠模型的疗效对比研究是本人在成都中医药大学攻读硕士学位期间在导师指导下完成的，该论文的著作权归成都中医药大学所有。由于本人的导师系外校兼职导师，