不同品系鸡akirin2和mir-155的克隆与表达特性分析-cloning and expression characteristics analysis of different strains of chicken a kirin 2 and mir - 155.docxVIP

目录摘要..............................................................................................................................IVAbstract...........................................................................................................................V第1章绪论..................................................................................................................1Akirin基因的研究进展1Akirin基因总述1昆虫akirin的生物学作用31.1.3脊椎动物akirin1的功能研究51.1.4Akirin2在高等脊椎动物中的生物学功能5Akirin2在免疫中的价值7鸡akirin2研究现状7microRNA与免疫8microRNA的生物合成和调控机制8microRNA的生物学功能9microRNA在免疫中的功能91.2.4miR-155的研究进展10Akirin2、NF-κB与miRNA的关系111.4选题的目的和意义.............................................................................................12第2章鸡akirin2基因的免疫功能研究142.1实验材料...........................................................................................................142.1.1实验动物.......................................................................................................142.1.2新城疫疫苗...................................................................................................142.1.3引物...............................................................................................................142.1.4酶和生化制剂...............................................................................................152.1.5主要仪器设备...............................................................................................152.2实验方法...........................................................................................................152.2.1雏鸡免疫.......................................................................................................15总RNA的提取15RT-PCR16PCR产物电泳182.2.5电泳结果的测量与数据分析.......................................................................182.3实验结果...........................................................................................................182.3.1总RNA提取结果182.3.2免疫后7天akirin2半定量表达分析182.3.3免疫后14天akirin2半定量表达分析192.4讨论......................................................