布洛芬抗兔血吸虫病肝纤维化的实验分析-experimental analysis of ibuprofen on rabbit schistosomiasis liver fibrosis.docxVIP

布洛芬抗兔血吸虫病肝纤维化的实验研究瀚姜目的：探讨布洛芬对兔日本衄吸虫瘸肝纤维化形成的影响及作用机制。方法：32只键痰瑟嚣兰兔，8只孛#为正常对照组，24只建立兔盔蔽囊病_|l于纾维讫模型。造模成功后所有感染兔均予吡喹酮杀虫，然后按性别、体重配对后随机分为三组，分为布洛芬治疗组，模型对照组，秋水伯碱对照组，治疗8周；采用放射免疫分静千法检测血清HA、PCIII，肝组织石螨切片作I、III裂胶原酱味酸天狼猩红染色，免疫组化SABC法检测野缀缓石蜡切片I、III裂胶原、TGF一01，原位杂交技术检测胖组织冰冻切片I、III型胶原、MMP～1、TlMP—lmRNA，劳应用计舞枫彩像病理图象分摄系统进行图象分掇。结果：实验结果显示，采用布洛芬治疗8周，布洛棼治疗组与模型对照组相比较，布洛芬治疗缝纛潺HA、Pel驳水平较模型缝嚣藩洚绦P固。05)，差雾骞显著瞧；菠藤特豫染魏_毒洛芬治疗组I、III型胶原沉积减少(P0．05)；免疫组化染色显示布洛芬治疗组肝组织I、III鼙蔽蒙沉积减少(P稍．05)，筠蓼一131表遮减少(PG．01)；原经杂交染惫显示布洛芬治疗组I、III型胶原mRNA、11MP一1nlRNA表达减少，MMP--lmRNA表达增加(P《o．05)。布洛芬治疗缀与欷永馈碱治疗组相眈较各项稔测指菰麓异不黧著(Po．05)。结论：布洛芬可抑制兔血吸虫病肝纤维化的发生发展，具有抑制兔脬组织l、III型胶原沉积、TGF--母1表达和HMP一1表达的作用，增加MMP—lmRNA表达。关键词：愈吸虫瘸翳纾续化农洛芬免疫缀佳原像杂交StudyonbrufenagainstschistosomiasiswithhepaticfibrosisinrabbitsHuangQiaoxin，TianDeying．DepartmentofInfectionsDiseases，TongjiHospitalTongjiMedicalCollege，Wuhan430030AbstractObjectiveToprobetheeffectandmechanismofbrufenonhepaticfibrosisofSchistosomiasisjaponica。MethodsModdsofSchistosomiasisrabbitswithhepaticfibrosisweresetuD．A11infectedrabbitsweregivenonetimeofgastriclavagebypyquitonandrandomlydividedintothreegroupsbysexandweight．TreatedgroupwasgivencolehicinesandnoHnalsalinerespectively．Agroupofnornlalrabbitswasusedascontr01．SerumHAandPC111weredetectedbyradioimmunoanalysisParaffin．embeddedlivertissuesectionswerestainedwithbitteracid．Immunohistochemistryspreptavidin-biotin．pemxidasecomplexmethod(smc)wasusedtodetectDpeICollagen(ColI)、TypeIIICollagen(C0lIlI)andTGF-p1．InsituhybridizationwasusedtodetectColImRNA、ColⅡImRNA、MMP．1mRNAand11MP．1mRNAinfrozensections．Computercolorinloathologicimageanalysissystemwasusedtoo．ResultsComparingthebrufentreatedgroupwiththecontrolgroup：(1)ThelevelofserumHAandPc111wasdecreasedO0．05)．(2)nedepositofhepaticColIandColIIIWasdecreased(p0．05)andtheexpressionof1’GF-p1wasweakenedO0．01)．(3)neexpressionofColImRNA、Col／IImRNAandTIMP—lmRNAwasdecreasedbutMMP．1mItNAincreasedfp0．05)．Thedmrencesbetweenbmfentreatedgroupandcolchicinetreatedgroupwerenotsignificant．CondusionBmfencouldresisttheoccurren