变应性鼻炎外周血th17 cd4il-10treg th12细胞功能研究-study on th17 cd4il - 10 treg th12 cell function in peripheral blood of allergic rhinitis.docxVIP

HC, DE were lower than AR （P＜0.05＝. After stimulated by mite, AR was decreased to blank on the Treg cell of IL-10+ （P＜0.05）. Conclusions: It suggested that Th17 cells maybe had an influence of AR.PMA could increase the percent of Th cells secreting IL-17. It demonstrated the Treg cell secreting il-10 has a role of immunologic mechanism on allergic rhinitis. Key Words: AR；DE；Der p1；PMA；Th17；Treg；IL-10  Function research of Th1/ Th2 cells of peripheral blood in AR and DE Abstract Objective :To investigate the Function of Th1/2cells of peripheral blood in the patients of allergic rhinitis and Desensitization treatment effective. Methods:10 patients of severe perennial allergic rhinitis(AR)due to mite ， 10 healthy controls(HC)andDesensitization treatment effective(DE) were enrolled.peripheral blood mo nonuclear cells(PBMCs) were collected, respectively divided into three groups: ionomycin group(blank group) ,adding ionomycin 1ug/ml and monesin 2ug/ml; mite group, adding ionomycin 1ug/ml; mite allergen nderp1 1ug and monesin 2ug/ml; PMA group , adding PMA 20ng/ml; ionomycin 1ug/ml and monesin 2ugl/ml. Cells were activated for 6h in carbondioxide cell incubator under 37℃ ， 5% CO2.Then it was continuous to do intracellular and membrane staining with PBMCs and survey the percent of th1/2 cell in CD4+ cell by flow cytometric detector, and analyze the function of Th1/2 cells in the patients of AR and DE. Probe the percent of th1/2 cell in CD4 t cell. Results: blank group,the percents of memory Th1 cell of HC was lower than that of AR, Mite groups were both decreased,but had no significantly. After the stimulated by PMA, the percent of Th1 cell in HC, AR and DE were higher than blank group（P＜0.05）. Blank group,the percents of memory Th2 cell of HC was higher than that of AR and DE, Mite groups were both had no significantly ,but had no significantly. After the stimulated by PMA, the percent of Th1 cell in HC, AR and DE were higher than blank group（P＜0