海洋微生物和微藻中防污活性物质的提取及其性能分析-extraction and performance analysis of antifouling active substances from marine microorganisms and microalgae.docxVIP

万方数据万方数据15.1μg·mL-1。红外光谱、高分辨质谱等的分析结果表明提取到的活性物质为一系 列的饱和、不饱和的脂肪酸，GC-MS进行结构检测，鉴定的物质为棕榈酸、硬 脂酸、二十碳五烯酸。（3）盐藻在制药、化妆品、营养剂、水产养殖饲料等方面有广泛的应用， 但盐藻代谢物的潜在防污性能尚未见报道。本文通过一系列萃取程序对其防污能 力进行探究，测试生物为中肋骨条藻和纹藤壶幼虫。盐藻的乙酸乙酯粗提物抑藻 活性是显著的，对硅藻的半数抑制率浓度72h-EC50值为 58.9 μg·ml-1。对乙酸乙 酯粗提物进行逐步提纯得到最后的提纯物B，该提取物对中肋骨条藻的72h-EC50 值为21.2 μg·mL-1，对纹藤壶幼虫的半数致死率24h-LC50值为18.8 μg·mL-1。红外 光谱、高分辨质谱、GC-MS等分析表明防污活性物质为一系列饱和、不饱和的 16-C、18-C脂肪酸，主要为棕榈酸、7,10-十六碳二烯酸、4,7,10-十六碳三烯酸、4,7,10,13-十六碳四烯酸、硬脂酸、亚油酸、亚麻酸。 以上研究结果说明海洋生物膜中微生物和海洋浮游藻均可产生具有较高防污活性的脂肪酸，有希望成为替代有毒防污剂的新型环境友好型防污剂的来源。关键词：生物污损、天然产物、生物膜、微藻、防污活性IIExtraction and performance study of antifoulingcompounds produced by marine microorganism and microalgaeAbstractIn the marine industry, the accumulation of living organisms on artificial surfaces by adhesion, growth, and reproduction is known as marine biofouling. With the advances in modern technology, human development and utilization of the oceans has made significant progress, and biofouling become thorny issues need to be resolved, therefore, the antifouling technology has obtained the widespread application. Antifouling coating is the most nature, economical and widely used method among all the solutions proposed the history of navigation. The traditional antifouling paints, including organic tin, cuprous oxide antifouling agents, cause serious pollution of the marine environment. With the gobal ban of application of organotin-based marine coatings by International Maritime Organization in 2008, there is a practical and urgent need of identifying environmentally friendly low-toxic and non-toxic antifouling compounds for marine industries. Marine natural products have been considered as one of the most promising cources of antifouling compounds in recent years. To meet various needs, a variety of effective and environmentally friendly AF compounds from natural sources have been developed and adopted in both research and commercial laboratories. In this paper, the antifouling compounds were isolated and extracted fro