环磷酰胺对大鼠脑I2fR损伤的保护作用及海马GFAP表达的影响.pdfVIP

Effect on the protection and the exression of GFAP of astrocytes in hippocampi treated by Cyclophosphamide after cerebral ischemia-reperfusion injury in rats Abstract Objective : To explore the neuro-protection and neuro-protective mechanism of cyclophosphamide by studing its effect on expressions of glial fibrillary acidic protein(GFAP), tumor necrosis factor-alpha(TNF- α )and neuroglobin(Ngb) in hippocampi and the survive of neuro after local cerebral ischemia-reperfusion injury in rats. Methods: Seventy healthy male Sprague-Dawley rats which avoirdupois from 180g to 220g provided by experimental animal department of South China University were randomly devided into 4 groups：normal control group(N group) ，sham operation group(S group) ，model control group (model group)and cyclophosphamide treated group(T group).Each groups had 18 rats,each of the later groups (S group，model group and T group) were randomly divided into 3 subroups by three time spots.Each of subgroup had 6 rats.The model of middle cerebral artery occlusion (MCAO) by improved suture embolus was established according to Longas report. Treatment group was given intraperitoneal injection of cyclophosphamide one hour later after ischemia;while both I/R group and sham operation group received same doses of saline.The formation of reperfusion following cerebral ischemia two hours later；each group was observed according appointed time spots—24 hours, 72 hours and 5 days.The scores of behavior obstacle in rats were rated by Longas report at different time after reperfusion, and then executed them to obtain the brains.Normal group were 5 executed and obtained brains.All the brains were formed by paraffin section.HE staining and immunohistochemistry staining were used to observe pathological changes and the expression of GFAP、TNF- αand Ngb