毛茛总苷对大鼠肾性高血压的影响及有效部位分析-effect of total glycosides of ranunculus ternatus on renal hypertension in rats and analysis of its effective parts.docxVIP
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毛茛总苷对大鼠肾性高血压的影响及有效部位分析-effect of total glycosides of ranunculus ternatus on renal hypertension in rats and analysis of its effective parts
SectionⅡTheeffectoftotalglycosidesofranunculusjaponiucusoncalciumionslevelinvascularsmoothmusclecellsofratsObjective:Toobservedtheeffectoftotalglycosidesofranunculusjaponiucusoncalciumionslevelinvascularsmoothmusclecells.Method:WeusedanewgenerationsofCa2+fluorescentprobe(Fluo-3/AM)tomarkcalciuminvascularsmoothmusclecellswhichwecultured.Observedthefluorescenceimagingbyuseinvertedfluorescencemicroscopeandmeasuredthefluorescenceintensityofcalciuminvascularsmoothmusclecellsbyusefluorescencespectrophotometer.Toobservedtheeffectoftotalglycosidesofranunculusjaponiucusoncalciumionslevelinvascularsmoothmusclecellsofrats.Results:TheangiotensinⅡcansignificantlypromotethecalciumionsinextracellularinnerflow.ThedifferentdosesoftotalglycosidesofranunculusjaponiucuscanreducethecalciumionslevelincellswhichmediatedbyangiotensinⅡ.Theresultsisrelatedthedosesoftotalglycosidesofranunculusjaponiucus.Conclusion:TotalglycosidesofranunculusjaponiucuscanantagonizetheeffectofAngⅡwhichcansignificantlypromotethecalciumionsinextracellularinnerflowpartⅡTheeffectivepartoftotalglycosidesofranunculusjaponiucusObjective:Tostudytheeffectpartoftotalglycosidesofranunculusjaponiucusonvasodilatationinvitro.Method:Werecordedthetensionofthoracicaorticinvitroandobservedtheinhibitioneffectofdifferentpartsoftotalglycosidesofranunculusjaponiucusoncontractionofthoracicaorticwhichmediatedbynorepinephrine(NE)andKCI.AstheRutintothestandardanduseUVspectrophotometertomeasurethetotalflavonoidcontentindifferentpartsoftotalglycosidesofranunculusjaponiucus.MakethecorrelationregressionoftheflavonoidcontentasabscissaandtheinhibitionratesasverticalcoordinatebyLogisticandlinearstatistics.Results:(1)ThedifferentalcoholpartoftotalglycosidesofranunculusjaponiucuscaninhibitionthetensionincreasedofthoracicaorticinvaryingdegreeswhichmediatedbynorepinephrineandKCI(P0.05).(2)Full-wavelengthscanningtodeterminethemaximumabsorptionwavelengthis358nm.TherelativestandarddeviationprecisionofexperimentsandstabilityexperimentRSDis1.00%.Theave
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