三种美白成分对牛牙表面茶蛋白质色渍清除效果评价-evaluation of the effect of three whitening ingredients on removing protein stains from bovine tooth surface tea.docxVIP

三种美白成分对牛牙表面茶/蛋白质色渍清除效果的评价[中文摘要]目的：考察不同浓度下的无花果蛋白酶（ficin,Fc）、过氧化脲（carbamideperoxide,CP）、和三聚磷酸钠(sodiumtripolyphosphate,STP)对牛牙表面茶/蛋白质色渍的脱色效果。方法：制备的牛牙釉质样本，经全唾液（wholesaliva,WS）、茶循环浸泡，建立着色模型；通过扫描电镜（scanningelectronmicroscope,SEM）观察样本着色前后表面的形态变化；用PR-655采集样本经不同浓度的Fc、CP、STP干预前后的色参数L*、a*、和b*，得到色参数变化量ΔL*、Δa*、Δb*及相应的ΔE*ab，并通过SEM观察样本表面的形态变化，最终得到Fc、CP、STP最佳安全有效浓度；比较最佳浓度下Fc、CP、STP之间的脱色效果，并分别通过接触角测量仪和SEM分析样本表面亲疏水性的改变和对釉质损害程度。结果：Fc对茶所引起的牙外源性着色有很好脱色效果（P0.05），随着浓度的增大，脱色效果不断增大，当浓度达到0.1%时，出现了肉眼可见颜色变化；CP、STP也有一定的脱色效果（P0.05）；在最佳浓度下Fc、CP、STP都具有一定的脱色效果（P0.05），其中Fc的脱色效果最好；此外牛牙釉质经三者干预后都没有出现明显的损伤。结论：在本实验条件下，Fc、CP、STP对茶所引起的牙外源性着色都有一定的脱色效果；但在最佳浓度下，Fc的脱色效果最好。关键词：无花果蛋白酶、过氧化脲、三聚磷酸钠、牛牙釉质、牙外源性着色、PR-6554Theevaluationofthreekindsofwhiteningingredientsremovalefficacyontea/proteinstainonbovinetoothsurface[Abstract]Objective:Toinvestigatethestainremovalefficacyofficin(Fc),carbamideperoxide(CP),andsodiumtripolyphosphate(STP)tohumanwholesaliva(WS)/teacomplexlayersonthebovineenamelsurfaceunderdifferentconcentrations.Methods:Tea/proteinstainsonbovineenamelwerecreatedbycyclingspecimensthroughhumanwholesalivaandteasolution.TheFc,CP,andSTPwereusedtoremovethestainfromtheenamelsurface.Inthisprocess,thescanningelectronmicroscope(SEM)wasintroducedtodetectchangesofthebovineenamelmicromorphology.ThecolorparametersofspecimensasL*,a*,andb*aswellasΔE*abweredeterminedbyusingspectrophotometerPR-655.ThebestconcentrationdataofstainremovalforFc,CP,andSTPwereobtainedbyusingSEMobservationofsurfacemorphologicchange.TocomparedecolorationefficacyofFc,CP,andSTPattheirbestconcentration,respectively,andtoanalyzethehydrophilcityandhydrophobicitychangesandextentofdamageonthesurfacesofspecimensbymeansofcontactanglemeterandSEM.Result:ThestainremovalefficacyincreasedwiththeconcentrationofFcincreasingwithinacertainrange(P0.05).Whentheenzymeconcentrationincreasedby0.1%,thechangeintotalcolourcanbeobservedwitheyes.AuthoughtthestainonbovineenamelcanberemovedbyCP,STPtosomeextent(P0.05),andthestainremovalefficacyisnotobvious,andthechangeintotalc