三种重金属单抗的制备与抗体基因序列研究及其免疫检测方法的建立-preparation of three heavy metal monoclonal antibodies and study of antibody gene sequence and establishment of immune detection method.docx

下载文档 降价啦

2
0
约13.7万字
约 143页
2018-07-30 发布于上海
举报
版权申诉
保障服务

三种重金属单抗的制备与抗体基因序列研究及其免疫检测方法的建立-preparation of three heavy metal monoclonal antibodies and study of antibody gene sequence and establishment of immune detection method.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

三种重金属单抗的制备与抗体基因序列研究及其免疫检测方法的建立-preparation of three heavy metal monoclonal antibodies and study of antibody gene sequence and establishment of immune detection method

0.257μg/mLand0.628μg/mLrespectively.Thecross-reactivitieswerelowerthanothermetalions.ThevariableregiongenesoftheMcAbsagainstmercury,copperandzincrespectivelywereamplifiedfrom3G4-G5,9D11-F3andZ1A5.Withtheoptimizationofworkingparameter,thestandardcurvesofIC-ELISAformercury,copperandzincwereestablished.Thedetectionrange(IC20~IC80)was0.15μM~9.0μM,0.055μg/mL~1.198μg/mLand0.062μg/mL~6.341μg/mLrespectively.Thelowestdetectionlimitwas3.94×10-5mM,0.0227μg/mLand0.015μg/mLrespectively.Recoveriesofmercury,copperandzincfromtheanalyte-fortifiedintotapwaterandultrapurewaterwereintherangeof88.9%~107.3%,87.4%~106.8%and88.6%~109.4%respectively.Thesynthesisofantigenofmercury,copperandzincwassuccessful.ThreehybridomacelllinesproducingstablyMAbswithhighaffinityandspecificityagainstmercury,copperandzincwereobtained.ThevariableregiongenesoftheMcAbsthatareagainstmercury,copperandzincrespectivelywereamplified,whichlaidafoundationfortransformationantibodywithgeneticengineering.TheIC-ELISAformercury,copperandzincwasestablished.Theimmunoassaysmethodsetthestageofthedevelopmentofimmunoassaytestkitsfordeterminingresiduesofmercury,copperandzinc,whichcouldprovideanimportanttoolforfarmproductsaswellasenvironmentalsafetytest,soitwouldhavetherealisticsignificancetoimprovetheefficiencyandqualityofriskassessment.KeyWords:Heavymetal;Mercury;Copper;Zinc;ChelateantigenMonoclonalantibody;Sequenceanalysis;IC-ELISADirectedby:Prof.WANGFenglong,AssociateResearcherLIXiaApplicantforDoctordegree:ZHAOLi(veterinarypathology）(AnimalScienceandAnimalMedicine.InnerMongoliaAgriculturalUniversity.Hohhot010018.China)目录1引言11.1重金属污染状况研究进展21.1.1重金属对水的污染21.1.2重金属对大气的污染21.1.3重金属对土壤的污染31.2重金属汞的污染及危害41.2.1重金属汞污染41.2.2重金属汞危害51.3重金属铜的污染及危害71.3.1重金属铜污染71.3.2金属铜危害81.4重金属锌的污染及危害91.4.1重金属锌污染91.4.2重金属锌危害101.5重金属污染的防治措施111.6重金属检测技术121.6.1重金属的常规性检测方法121.6.2重金属的免疫检测方法161.7重金属抗原和抗体制备技术研究进展201.7.1重金属人工抗原的合成201.7.2重金属抗体的制备221.8研究的目的和意义242重金属汞、铜和锌螯合抗原的制备与鉴定252.1材料与方法252.1.1材料252.1.2方法262.2结果302.2.1重金属汞人工螯合抗原的鉴定302.2.2重金属铜螯合抗原的鉴定342.2.3重金属锌螯合抗原的