桑树mcax-1和mrd22基因的克隆及序列与表达分析-cloning, sequence and expression analysis of mulberry mcax - 1 and mrd 22 genes.docxVIP

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桑树mcax-1和mrd22基因的克隆及序列与表达分析-cloning, sequence and expression analysis of mulberry mcax - 1 and mrd 22 genes.docx

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桑树mcax-1和mrd22基因的克隆及序列与表达分析-cloning, sequence and expression analysis of mulberry mcax - 1 and mrd 22 genes

Abstract：As a first step towards characterization of functional genes in mulberry,in this paper,we constructed a cDNA library from young mulberry leaves.Based on the research above,we cloned two important genes and carried out the analysis of their sequences.Furthermore,we conducted the functional verification of the two genes by the means of stress-induced method based on the prediction of their functions.The main results were summarized briefly as follows: Molecular cloning and sequence analysis of Calcium Exchanger Gene,MCAX-1,in Mulberry (Morus alba L.) Calcium exchanger play an important role in Ca2+-mediated nutrition and signal transduction in plants.The full-length cDNA that is cloned through RACE and RT-PCR methods,named as MCAX-1(Genbank accession No.JN716318),cDNA fragments of which has been found by measuring the function comments of ESTs sequence in mulberry,is 1784bp by the sequence analysis.It contains a 197bp 5-UTR (untranslated region) and a 243bp 3-UTR,an open reading frame (ORF) of 1344bp,encoding 447 amino acids with a predicted molecular weight of 47.93kD and an isoelectric point of 5.67.A phylogenetic analysis revealed that mulberry had close genetic relationship with Suaeda salsa,Populus trichocarpa and Ricinus Communis.Semi-quantitative RT-PCR analysis showed that the transcription levels were relatively higher in top bud,young leaf,root and young bloom,relatively lower in phloem,xylem and mature fruit;gradually reduced in the low temperature stress,almostly no expression at -1℃,gradually risd to the maximum then straightly reduced in the drought stress,nearly not changed in the early of salt stress,but significantly reduced on the 18 days.It initially speculated that MCAX-1 and the resistance of mulberry have some relevance. Molecular cloning and sequence analysis of Dehydration-responsive Protein Gene, MRD22,in Mulberry (Morus alba L.) EST encoding RD was isolated from the cDNA library.And its full open reading frame were ob