乳腺癌转移抑制基因brms1功能的分析-analysis of the function of breast cancer metastasis suppressor gene br ms1.docx

下载文档 降价啦

2
0
约7.82万字
约 108页
2018-07-30 发布于上海
举报
版权申诉
保障服务

乳腺癌转移抑制基因brms1功能的分析-analysis of the function of breast cancer metastasis suppressor gene br ms1.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

乳腺癌转移抑制基因brms1功能的分析-analysis of the function of breast cancer metastasis suppressor gene br ms1

StudyonBiologicalFunctionsofBreastCancerMetastasisSuppressor1（BRMS1）AbstractObjectiveToinvestigatetheanti-canceractivitiesofbreastcancermetastasissuppressor1(BRMS1)intwoinvasivebreastcancercelllines.Method1．BasedontheBRMS1genesequencefromtheGenebank,afull-lengthBRMS1cDNAwasamplifiedbyapairofspecificallydesignedprimersandconstructedintoapcDNA3eukaryoticexpressionvector.2．CelllineswithhighexpressionofBRMS1wereestablishedbystabletransfectionofpcDNA3-BRMS1expressionunderthehelpofcationicliposome(Lipofactamin2000)andselectioninmediumcontainingG418.3．TheeffectofBRMS1overexpressiononcellgrowthandproliferationwereobservedusingMTTmethod.4．CellmigrationandinvasionweredeterminedbyinvitroscratchandmodifiedBoydenchamberassays.5．Alterationofcellcycleprogressionandapoptosisinductionwerestudiedbyflowcytometry.6．BRMS1roleonthetumorgrowthandmetastasisinvivowerestudiedinnudemicewithorthotopicinjectionofbreastcancercells.7．TheeffectsofBRMS1onlungmetastasiswasobservedviatailintravenousinjectionofbreastcancercells.8．Radiosensitivitywasanalyzedbycolonyformingassays.9．AnalysisofchromosomalaberrationsandPCCfragmentswereperformedtodeterminetheeffectofBRMS1overexpressionongenomicinstability.10．Westernblotassaywasusedtomeasureproteinexpression.11．InvitroGSTpull-downandinvivoimmunoprecipitation-WBassayswereemployedStudyonBiologicalFunctionsofBreastCancerMetastasisSuppressor1（BRMS1）Abstracttodetermineprotein-proteininteraction.12．LuciferaseassaywasusedtodeterminetranscriptionalactivityResults1．ConstructionoftheBRMS1eukaryoticexpressionvectorwasconfirmedbysequencinganddigestionwithrestrictionendonucleasesBamHIandXhoI.2．CharacterizationofG418resistanceclonebyRT-PCRandWesternblotindicatesthatBRMS1over-expressingcelllineshavebeenestablished.3．EctopicexpressionofBRMS1didnotproduceanyeffectsoncellgrowthinbothMDA-MB-231andMDA-MB-435.4．EctopicexpressionofBRMS1didnotaltermigrationabilityofMDA-MB-231andMDA-MB-435cells,butsignificantlyreducedcellinvasion.6．BRMS1overexpressiondidnotcauseanyeffectsoncellcycl