双歧杆菌灌胃对5-fu腹腔注射大鼠肠屏障功能的影响-effect of bifidobacterium on intestinal barrier function in rats after intraperitoneal injection of 5 - fu.docxVIP

结论 1. 腹腔注射 5-Fu 能成功建立大鼠小肠黏膜损伤-修复模型，大鼠表现出腹 泻次数和腹泻量的增加及相应的体重下降，小肠形态学改变表现为绒毛坏 死，隐窝消失，肠屏障功能改变表现为机械屏障功能受损（肠黏膜 Occludin 蛋白表达减少）、肠免疫屏障受损（肠黏膜 sIgA 含量下降），同时肠上皮 再生修复能力下降（PCNA 含量下降）。5-Fu 500mg/kg 一次性腹腔注射大鼠 在停药 1 周后肠粘膜损伤可以逐步修复。 大鼠 5-Fu 腹腔注射连续五天的给 药方式较一次性给药方式所造成的肠粘膜损伤更为严重。 2. 长双歧杆菌灌胃能在一定程度上减轻5-Fu腹腔注射大鼠小肠粘膜结构的 破坏，减轻腹泻症状，减轻体重丢失；能增加5-Fu腹腔注射大鼠小肠粘膜 Occludin蛋白及sIgA表达；能增加化疗损伤期大鼠小肠粘膜的PCNA含量，促 进肠上皮损伤的修复。 关键词 肠屏障功能 双歧杆菌 化疗 Abstract Objective: Observe the intestinal barrier function of SD rats treated with 5-Fu intraperitoneal injection , compare the effect of different scheme of 5-Fu administration on the intestinal barrier. Investigate the role of Bifidobacterium longum in the protection of the intestinal barrier function in such situation. Method： 72 SD rats were divided into six groups, each group with 12, they were Control group of low-dose(A), 5-Fu in low dose (B), Bifidobacterium longum with low dose 5-Fu (C), Control group of high-dose(D), 5-Fu in high dose (E), Bifidobacterium longum with high dose 5-Fu (F). Low dose groups daily injection 5-Fu 40mg/kg intraperitoneal D1-5, high dose groups injection 5-Fu 500mg/kg intraperitoneal on the first day, control groups inject saline instead. Bifidobacterium longum were given to group C, group F orally D1-7,other groups were given saline instead. The weight and diarrhea were data everyday. On the fourth day and on the eighth day killed 6 rats in each group , to investigate the pathological changes of intestinal mucosa and the expression of Occludin, PCNA, sIgA. Results: Weight Rats in group A and group D body weight increased daily while others loosed body weight continuously. On the fourth day the weight lose of rats in group B,C,E,F was significantly compared with control groups P0.05, the weight loss of rats in group C was less than other three groups P0.05. On the eighth day, weight of rats in group B,C,E,F dropped further, the weight loss of rats in group F less than other three groups P0.05. Diarrhea On the fourth day ,Rats in gro