山核桃外植体诱导培养及褐化机理的初步分析-preliminary analysis of induction culture and browning mechanism of carya cathayensis explants.docx

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2018-07-31 发布于上海
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山核桃外植体诱导培养及褐化机理的初步分析-preliminary analysis of induction culture and browning mechanism of carya cathayensis explants.docx

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山核桃外植体诱导培养及褐化机理的初步分析-preliminary analysis of induction culture and browning mechanism of carya cathayensis explants

PAGE PAGE III Abstract In this study, researching on the tissue culture of Caryacathayensis Sarg. by the follow methods,such as selective medium,sterilization methods and anti-browning program.And combined with the explants biochemical markers and cell slice observation to study of the tissue culture and browning mechanism of Carya cathayensis Sarg.And provided a theoretical basis for accelerated Carya cathayensis Sarg. breeding , improved the varieties of seedlings.The results are as follows. In the selection of the best explants, stems with buds was the best explants, it can generate callus and the bud also germination, the average rate of callus was 86.43%, the average rate of sprouting is 45.29%. The optium medium was WPM,The average browning rate of WPM was 40.35%, and the average germination rate was 45.34%. The best method of sterilization was to soak the explants in 1000 times of carbendazim for 30 mins, 75% alcohol for 60s, 0.1% mercuric chloride for 10mins .In this method,the average rate of contamination was 26.21%, the average rate of germination was 44.91%. The best method of sterilization was put 200 mg/L penicillin sodium into the medium，the average rate of germination was 7.80%. After the anti-oxidant added into the medium, Vc100mg/L+ Na2S2O3 0.2mg/L+ AgNO3 0.10mg/L+PVP 0.1 mg/L has a good effect of anti-browing,the germination rates was 22.23%,and the average rate of germination was 43.80%. Nature anti-oxidant has a good effevt of anti-browing.After added 3.0g / L of tea into the medium,the average browning rate was 31.59%.And the average rate of germination was 46.23%. 3.0g/L tea with 0.10g/L AgNO3 has a good effect of anti-browing.,the average browning rate was 23.07%.The average germination rate was 45.47%. In the selection of dark and cold culture environment, 2 days of 10 ° C dark culture has an effect of anti-browning,the average browning rate was 21.24%,the average germination rate was 46.07%. The optimal auxin typ