石蒜不定根培养体系的建立及石蒜生物碱成分的分析-establishment of adventitious root culture system of lycoris radiata and analysis of alkaloids in lycoris radiata.docxVIP

accumlatedinepidermis,cortexandvascularbundles;inthebulbs,theepidermis,abaxialparenchymacellsandvascularbundlesarethemainpartsoftheaccumulationofalkaloids;intheleaf,distributionofalkaloidsaresimilartochloroplastdistribution,mainlyincortexandvascularbundles.Thus,alkaloidsmainlydistributedinthevascularcylinderofroots,bulbsandleaves,especiallyinthesecellwalls.4.Two-dimensionalthin-layerchromatography(2D-TLC)wasusedtoseparatedandpreparedalkaloid.Thebestseparationwassolvent1EtOAc:MeOH:NH3·H2Oas9∶11∶0.65andsolvent2CHCl3:MeOH:NH3·H2Oas18∶2∶0.5in2D-TLC,Two-dimensionalthinlayerchromatographycanseparate19kindsofalkaloids,andthesealkaloids’purityishigher.SomealkaloidsanalyzedbyIR,oneofwhichisN-Methylnicotinamide.Keyword:Lycorisradiat,alkaloid,rootculture,vegetativeorgans,histochemicallocalization,2D-TLCIII目录摘要.............................................................................................................................................IAbstract......................................................................................................................................II目录...........................................................................................................................................IV1文献综述.................................................................................................................................11.1石蒜简介......................................................................................................................11.2植物组织培养..............................................................................................................11.3根的组织培养..............................................................................................................21.4石蒜的组织培养.........................................................................................................31.5石蒜生物碱..................................................................................................................41.6生物碱的合成途径研究........