石墨烯-银纳米复合材料的制备及性能分析-preparation and properties analysis of graphene - silver nanocomposites.docxVIP

摘要更有效地与细菌接触，因此使GO-Cys-AgNPs复合材料的抗菌性能相比于GO-AgNPs有显著提升。同时，将Cys-AgNPs与GO在较高温度下进行复合，制备的rGO-Cys-AgNPs作为电极修饰材料来修饰玻碳电极，得到的三电极尿酸电化学传感器具有较好的灵敏度，线性检测区间为0.025μM-50μM(R=0.997)和200μM-2250μM(R=0.999)，检测极限为0.025μM(S/N=3)，可能是石墨烯大的比表面积和银纳米粒子良好导电性之间的协同作用。关键词：石墨烯银纳米粒子抗菌性能电化学性能IIAbstractGraphenehasemergedasthemostimportantplatformforcompositefabricationduetoitsunique2Dsingle-atomstructure,largerspecificsurfacearea,excellentadsorptionproperty,biocompatibilityandchemicalstabilityproperties.Graphene-basednanocompositematerialshavebeenstudiedextensivelyintheoptics,biomedicine,sensors,storage,andotherfields.Ontheotherhand,Agnanoparticles(AgNPs)havereceivedmuchattentionintheareasofphotonics,photography,catalysis,sensor,biomedicineandsurfaceenhancedRamanscattering(SERS)becauseoftheiruniquephysicalandchemicalproperties.Inthiswork,graphenehasbeencombinedwithAgNO3throughdifferentmethodstofabricatetheGO-AgNPsnanocomposites,andtheantibacterialactivityandelectrochemicalpropertyoftheresultingnanocompositeswerestudied.In-situmethod.TheGO-AgNPsnanocompositeswerepreparedinanone-potandgreenapproachinalkalinesolution,withoutanyreducingagentorsurfactant,usingAgNO3asprecursorandGOassubstrate.Factorsthataffectthephysicochemicalpropertiesofthenanocomposites,suchaspH,reactiontemperature,reactiontimeandtheratioofAgNO3/GO,wereexploredtoobtainoptimumreactionconditions.Underoptimumreactionconditions(pH=11.7,WAgNO3:WGO=1:1,at60℃for30min),thesmall-sized(ca.15nm)andhighydispersedAgNPsweresupportedonGOsheetsandtheobtainednanocompositesexhibitedanexcellentwater-dispersibility.TheantibacterialpropertiesofGO-AgNPsweretestedagainstGramnegativebacterialstrainEscherichiacoli(DH5α)byacolonyformingcountmethod.GO-AgNPsshowedagoodantibacterialactivitywith98.7%ofthecellsinactivatedattheGO-AgNPsconcentrationof20μg/mL.ComparedtoGO,theenhancedantibacterialeffectofGO-AgNPscompositescouldbeascribedtothesynergisticeffectofAgNPsandGO.Phasetransfermethod.AgNPsweresuccessfullypreparedintolueneusingNaBH4asreducingagentandtetraoctylammoni