厦门近海温泉微生物多样性及热稳定纤维素酶的分析-analysis of microbial diversity and thermostable cellulase in hot springs off xiamen coast.docx

InvestigationofmicrobialdiversityofthecoastalhotspringinXiamenandcharacterizationofthethermostablecellulasesAbstractTheenvironmentofhotspringwasquitesimilarwithearlyEarth,andtheecologyofmicrobialinhotspringwassimpleandstable.Theexplorationofmicrobialdiversityinsuchecosystemwasimportantfortheunderstandingofthemicrobialcommunitystructure,physiologyandtherelationshiptothegeochemicalconditions.Italsowashelpfulforunderstandingoftheoriginandtheevolutionoflife.Thermophilesweretheuniquemicrobialsourceandrarematerialofscientificresearch.Hotspringhadattractedbroadinterestbecauseoftheuniquethermophilicpropertiesoftheorganismsthrivinginthesebiotopesandthedescriptionofanincreasingnumberofnewthermophilicspecies.Thermostableenzymessynthesizedbythermophilicmicroorganismsweretheimportantresourcesinmanyindustrialprocessesandbiochemistry.Cellulasesplayedanimportantroleinthebiomassenergy,especiallythermostablecellulases.Inthestudy,thebacterialandarechaealdiversityofthecoastalhotspringinXiamenwereinvestigatedbyculture-independentmolecularapproaches.Three16SrDNAgenelibrariesofbacterialcommunityfromdifferenttemperaturehotspringsedimentswereconstructedandtheanalysiswereperformedbyamplifiedribosomalDNArestrictionanalysis(ARDRA)technique.ThesequencesimilaritieswereanalyzedbyusingtheBLASTprogramsforsearchingtheGenBankDNAdatabases.Thephylogeneticanalysisindicatedthatmostofthebacterial16SrDNAsequencesbelongedtoatleasteightgroups,includingProteobacteria,Aquificae,Bacteroidetes,Chloroflexi,Thermotogales,FirmicutesandAcidobacteria,andafewbelongedtounknowngroups.ThebacteriallibrariesweredominatedbyProteobacteria.Inhotspringof90℃,Alphaproteobacteriawasthepredominantspecies（37.5％）.WhileBetaproteobacteriawasdominantinthehotspringof70℃(43.9%)and80℃(50.6%)。Todescribearechaealdiversity,thearechaeal16SrDNAgenelibrariesofarechaealcommunityfromdifferenttemperature(70℃and80℃)hotspringsedimentswereconstructed.Thephylogeneticanalysisindicatedthatthearechaeal16SrDNAsequencesbelongedtoCrenarchaeotaandEuryarchaeo