乌饭树果实花色苷的提取及其抗氧化和抑菌特性分析-extraction of anthocyanin from vaccinium bracteatum fruit and analysis of its antioxidant and antibacterial properties.docxVIP

福建农林大学硕士学位论文乌饭树果实花色苷的提取及其抗氧化和抑菌特性研究Theanti-oxidationactivityofanthocyaninextractiveanditsdifferentpolaritiesofsolventsfromVacciniumbracteatumThunb.fruitswasstudied.Theresultsshowedthat:theanti-oxidationactivityofanthocyaninextractivewasweakerthanthepolaritiesofsolventsonthethreeanti-oxidationsystems.Theorderoftheactivityofscavengingon·OHwas:ethyl-acetatephaseascorbicacidn-butyl-alcoholphasechloroformphaseanthocyaninextractive,theorderoftheactivityofscavengingonO2-·was:ethyl-acetatephaseascorbicacidchloroformphase≈n-butyl-alcoholphaseanthocyaninextractive,andtheorderoftheactivityofinhibitinglipidper-oxidationwas:ethyl-acetatephasechloroformphase≈n-butyl-alcoholphaseanthocyaninextractiveascorbicacid.ThroughqualitativeandUV-Visabsorptionspectralcharacteristicsexperiment,theethyl-acetateextractionpartwasjudgedintoanthocyanin.Theanti-microbialactivityofethyl-acetatephasewasstudied,andthefactorsincludingtemperature,pH,time,andsoon,whichinfluencetheinhibitioneffectofSaccharomycescerevisiaewasdiscussed.Theresultsshowedthat:theethyl-acetatephasehadworseanti-activityforfungi,evenadvancedthegrowthofyeasts,suchasSaccharomycescerevisiae.Butithadthestrongeranti-activityforbacteria,thedegreeofanti-bacteriaactivitywas:EscherichiacoliBacillussubtilisStaphylococcusaureus,andtheminimalinhibitoryconcentrationwere0.625mg/mL,1.25mg/mL,1.25mg/mL.Theseveralfactorsalsohadsignificantlyeffectonanti-bacteria,theanti-microbialactivityofethyl-acetatephasewasstrongerinnormalorlowtemperatureandinneutralorpartialacidity;atthesametime,sugarorsaltcanimprovetheanti-microbialactivity.Keywords:VacciniumbracteatumThunb.fruits;anthocyanin;extraction;anti-oxidation;anti-microbial缩略词表英文缩写U英文全称Unit中文全称酶活力单位TACThetotalactivityofantioxidation总抗氧化能力BHABHTTrisButylatedhydroxyanisoleButylatedhydroxytoluene2-Amino-2(Hydroxymethyl)-1,.3-propanediol丁基羟基茴香醚丁基羟基甲苯三羧甲基氨基乙烷TBAThiobarbituricacid硫代巴比妥酸LLSL-a-Phosphatidylcholinesolution卵磷脂溶液PDAPotatodextroseagar马铃薯葡萄糖琼脂MICMinimalinhibitoryconcentration最低抑菌浓度MBCMinimalbacte