湘西杜仲有效成分提制分析及其指纹图谱分析-extraction and analysis of effective components of eucommia ulmoides oliv.in xiangxi and its fingerprint analysis.docxVIP

湘西杜仲有效成分提制分析及其指纹图谱研究 湘西杜仲有效成分提制分析及其指纹图谱研究 V V Selection and fingerprints of the control substances for plant drug Eucommia ulmodies Oliv by HPLC and LC-MS The selection of the control substances and their fingerprints of this plant medicine were investigated by using HPLC and LC-MS. The gradient elution mode was applied in chromatographic separation, and the data were analyzed by “Computer Aided Similarity Evaluation” software to compare the similarity of the E. ulmodies from different habitats. Low similarity was found in the samples from nonadjacent provinces, while high similarity was obtained in those from the adjacent provinces. The LC-MS fingerprints of E. ulmodies showed the main active constituents and could be used for its original identification and quality evaluation. Determination of elements in the E.ulmodies The E.ulmodies is a miraculous tree. Its leaves bark and flowers contain trace elements in human. The elements of the E.ulmodies were determined by FAAS. The results showed that: There were abundance elements, such as Mg Cu Fe, et al. The contents of them were higher in leaves than in bark, and they played a very important part in the metabolish of the flesh. While the contents of the element, such as Pb Cr, were very low. So E.ulmodies is a very safe Chinese traditional medicine. The experiment results provided the scientific basis for further research. Meanwhile by comparing different methods, the microwave digestion had the merits of efficiency, rapidity, less pollution and convenience. It is the best sample preparation method for determination plant’s trace elements contents. 4. Study on antibiotic ability of leaves extracts of E.ulmodies Chlorogenic acid was extracted from E.ulmodies by Microwave-Extraction method. The solvent was a mixed solution of ethanol and water. The antibiotic ability of chlorogenic acid under optimum extraction condition was studied. The result showed that, the extract had the antibiotic ability towards Escheric