调节性t细胞的浸润及tgf-β1 il-10的表达与人脑胶质瘤复发的相关性-correlation between the infiltration of regulatory t cells and the expression of tgf - β 1 il - 10 and the recurrence of human glioma.docxVIP

福建医科大学硕士研究生毕业论文 福建医科大学硕士研究生毕业论文 万方数据 万方数据 目录 全文略词··························································4 摘要······························································5 正文······························································8 前言······························································8 材料与方法························································9 结果······························································16 讨论······························································23 结论······························································26 参考文献··························································27 综述······························································31 参考文献··························································37 致谢······························································41 3 英文缩略词(Abbreviation) 缩略词 AP 英文全称 Alkaline phosphatase 中文名称 碱性磷酸酶 BCA BSA Bicinchoninic acid Albumin from bovine serum 2,2-联喹啉-4,4-二甲酸二钠 牛血清白蛋白 CD DAB ELISA Cluster of differentiation 3,3’-Diaminobenaidine Enzyme linked immunosorbent assay 分化群 3，3’－二氨基联苯胺 酶联免疫吸附剂测定 Foxp3 HE HRP Forkhead box Henatoxylin－eosin staining HYPERLINK /v5852416.htm?ch=ch.bk.innerlink Horseradish Peroxidase 前额盒P3 苏木素－伊红染色 HYPERLINK /ShowTitle.e?sp=S%E8%BE%A3%E6%A0%B9%E8%BF%87%E6%B0%A7%E5%8C%96%E7%89%A9%E9%85%B6 辣根过氧化物酶 IL-10 MHC OD Interleukin-10 Major histocompability complex Optical density 白介素-10 主要组织相容性抗原 吸光度 PBS PMSF TBS Phoshate Buffered Saline Phenylmethanesulfonyl fluoride Tris Buffer Saline 磷酸盐缓冲液 HYPERLINK /view/3853452.htm 苯甲基磺酰氟 三羟甲基氨基甲烷缓冲液 TGF-β1 TMB Tregs Transforming growth factor-β1 3,3,5,5-Tetramethylbenzidine Regulatory T cells 转化生长因子-β1 3,3,5,5-四甲基 HYPERLINK /view/150495.htm 联苯胺 调节性T细胞 4 中文摘要 目的: 检测胶质瘤组织中 CD4+FOXP3+调节性 T 细胞(Treg)浸润及 TGF-?1、IL-10 表达 情况,分析肿瘤组织微环境中三者的相互关系,探讨 Treg 浸润及 TGF-?1、IL-10 表达在不 同级别人脑胶质瘤组织复发的关系。 方法: 收集同一患者经手术切除并经病理科证实的初发、复发脑胶质瘤 26 例（患者均 排除炎症、自身免疫病），取每位患者 2 次术后组织标本共计 52 份分为初发组及复发组， 再按 2007 年 WHO 中枢神经系统肿瘤分类及分级标准，WHO I-II 级为低级别,