温度对郁金香鳞茎内源激素及芽体发育影响-effect of temperature on endogenous hormone and bud development of tulip bulb.docxVIP

青海大学硕士学位论文摘 青海大学硕士学位论文 摘 要 II II .不同温度处理郁金香鳞茎，低温处理下 SOD 4 周前活性较高，主要是低 温刺激所致，4~8 周生理活性相对较弱，呈现下降趋势，8 周后又开始上升并逐 渐达到稳定状态，这与休眠解除有关。POD 酶活在处理过程中变化幅度较小， 不能反映鳞茎休眠状态。 .不同温度处理郁金香鳞茎，0℃处理和 5℃处理芽体发育良好，出芽、开 花、植株高度、花径大小等指标均表现较好，所以 0℃和 5℃处理均可作为郁金 香品种“Golden Apeldoorn”促成栽培和切花生产的鳞茎处理温度。 关键词：郁金香 休眠鳞茎 温度 植物激素 芽体发育 青海大学硕士学位论文Abs 青海大学硕士学位论文 Abstract III III Abstract Tulip is a famous flower in the word, study the relationship between temperature treatments on physicohemical properties and bud development and bulbs dormancy is helpful the ornamental cultivation anniversary. Select 8-10cm bulbs of tulip cultivar Golden Apeldoorn and stored in room temperature(10-18℃), 5℃, 0℃and -12℃ and changing temperature. Test the changes of GA3、IAA、ABA by HPLC before treatment, 2nd week, 4th week, 6thweek, 8th week, 10th week, 12th week and 13th week during treatments. In addition test the changes of moisture, soluble sugar starch content，SOD and POD, meanwhile observe the bud development in each temperature under microscope before treatments, 4th week, 8th week, 12th week and 13th week during treatments separately. After 13 weeks stored, plant the bulbs in the green house then statistical emergence rate and flowering rate. The experiment result were as follows: 1.A method which was detected by high performance liquid chromatographic (HPLC) and extracted by isopropy alcohol solution for the simultaneous determ ination of three kinds of endogenous hormone gibberellin(GA3), auxin (IAA),and abscisic acid (ABA) content in tulip bulbs was descried. The chromatograpgic separetion was carried out on a reverse phase C18 colum using V（Met）:V（0.07% acetic acid） =45:55 as the mobile phase at a colum temperature of 20℃,the flow rate was 1.0 mL/min,UV detection at 212 nm was used for separation GA3 from 3min to 4.5 min, while detection at 218nm was used for separation IAA from 4.5min to 6.5 min, then detection at 265 nm was used for separation ABA at remaining time .The injection volum wasd 10μL.It