KLF2KLF4预测内毒素所致急性肺损伤大鼠研究.docVIP

KLF2KLF4预测内毒素所致急性肺损伤大鼠研究 　　【摘要】目的 研究Krüppel样转录因子KLF2、KLF4在内毒素诱导大鼠急性肺损伤时的动态表达，分析两者与急性肺损伤的相关性。方法 将100只SD大鼠随机（随机数字法）分为健康对照组和模型组，模型组进一步随机分成3组。模型组采用尾静脉注射LPS（5 mg/kg）复制ALI模型，分别于尾静脉注射后2、4、24 h后采血及肺组织。观察各组病理表现、RT-PCR检测KLF2 mRNA、KLF4 mRNA在血清中及肺组织的表达，分析KLF2、KLF4在急性肺损伤中的动态表达，采用SPSS 17.0软件进行统计学分析。结果 病理显示造模后4 h肺损伤最为明显，KLF2 mRNA、KLF4 mRNA在正常大鼠肺组织及血清中均有明显表达，模型组各组KLF2、KLF4的表达较对照组显著减弱（P0.01），其中KLF2 mRNA在2 h表达明显低于4 h组和24 h组（P0.01），KLF4 mRNA在4h表达明显低于2 h组和24 h组（P0.01）。结论 KLF2在大鼠急性肺损伤表达变化早于病理变化，KLF4具有同步性；KLF2、KLF4可作为急性肺损伤早期诊断的分子标志物。 　　【关键词】KLF2；KLF4；急性肺损伤；内毒素；早期诊断 　　Study the roles of KLF2， KLF4 in prediction of acute lung injury in rats induced by endotoxin Yang Yong， Shen Youkui， Dong Liwen， Lou Zhengqing， Wang Jun， Fu Xiaoqing. Department of Cardiothoracic Surgery， Guangxin Affiliated Hospital of Zhejiang Chinese Medical University， Hangzhou 310007，China 　　Corresponding author： Shen Youkui， Email： shenyoukui@126.com 　　【Abstract】Objective To investigate the expressions of KLF2 mRNA and KLF4 mRNA in the acute lung injury （ALI） rats induced by lipopolysaccharide （LPS）， and to analyze the correlation between KLF2， KLF4 and ALI.Methods A total of 100 SD rats were randomly divided into 2 groups： normal control group and LPS treated group， then the latter group was randomly further divided into 3 subgroups as per the serum and lung tissue samples taken separately at 2， 4 and 24h after modeling. The ALI model was made by injecting 5mg/kg LPS into tail vein .The pathological changes of lung tissue were observed in each group，and the expressions of KLF2 ，KLF4 mRNA in serum and lung tissue were detected by RT-PCR. The data of laboratory findings were analyzed with SPSS 17.0 software for statistical analysis. Results The histopathological changes showed the most obvious damage of lung tissue occurred at 4 hours after modeling. The expressions of KLF2 mRNA and KLF4 mRNA in the lung tissue and serum of control group were significantly higher compared to LPS treated subgroups （P 　　【Key words