C―erbB―2p―Akt蛋白在乳腺癌中表达水平及其在保留NAC乳腺癌手术中临床意义研究.docVIP

C―erbB―2p―Akt蛋白在乳腺癌中表达水平及其在保留NAC乳腺癌手术中临床意义研究 　　[摘要] 目的 探?C-erbB-2、p-Akt蛋白在乳腺癌中的表达水平及其在保留NAC乳腺癌手术中的临床意义。 方法 自2010年6月～2011年3月，随机选择86例经穿刺病理诊断确诊乳腺非浸润性导管癌，且符合保留NAC乳腺癌手术的女性患者为患者组。在我院健康体检中心随机选择86例健康女性为健康对照组。对患者组患者进行保留NAC乳腺癌手术，术后对癌组织及癌旁正常组织进行免疫组化染色。检测C-erbB-2、p-Akt蛋白在癌组织及癌旁正常组织中的表达水平。使用ELISA法对患者手术前后及健康对照组外周血的C-erbB-2、p-Akt表达水平进行检测，记录各组数据并进行统计学分析。按照C-erbB-2、p-Akt表达情况分为双阳性、双阴性以及单阳性。使用Kaplan-Meier法分析患者生存预后。 结果 在乳腺癌组织及癌旁正常组织中，C-erbB-2、p-Akt蛋白均有表达，但在癌组织表达水平明显高于正常组织，差异具有统计学意义（P0.05）。乳腺癌患者外周血C-erbB-2、p-Akt与健康对照组相比均明显升高。Kaplan-Meier法分析患者生存预后发现，C-erbB-2、p-Akt均阳性表达者生存率明显降低，总生存期明显缩短，差异具有统计学意义（P0.05）。 结论 乳腺癌患者外周血C-erbB-2、p-Akt水平明显高于健康对照组，C-erbB-2、p-Akt对患者接受保留NAC乳腺癌术后生存期有重要的临床意义。 　　[关键词] 乳腺癌；C-erbB-2；p-Akt；乳头乳晕复合体 　　[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-9701（2017）12-0001-04 　　[Abstract] Objective To investigate the expression level of C-erbB-2 and p-Akt proteins in breast cancer and its clinical significance in breast cancer surgery reserving NAC. Methods From June 2010 to March 2011， 86 patients who were diagnosed with noninvasive ductal carcinoma of the mammary gland by biopsy pathological diagnosis and eligible for breast cancer surgery reserving NAC were randomly selected as the patients group. Another 86 healthy women were selected as the control group， randomly selected from the Physical Examination Center in our hospital. The enrolled patients were given the breast cancer surgery reserving NAC. Immunohistochemical staining was performed on cancer tissues and adjacent normal tissues after the surgery. The expression levels of C-erbB-2 and p-Akt in cancer tissues and adjacent normal tissues were detected， and the expression levels of C-erbB-2 and p-Akt in the peripheral blood before and after the surgery and in the healthy control group were detected by ELISA. The data of each group were recorded and analyzed statistically. According to the expression of C-erbB-2 and p-Akt， the expression was divided into double positive， double negative and single positive. Kaplan-Meier method