miRNA―1和急性心肌梗死后心肌缺血程度相关性研究.docVIP

miRNA―1和急性心肌梗死后心肌缺血程度相关性研究 　　[摘要] 目的 研究循环miRNA-1和急性心肌梗死后心肌缺血程度的相关性。方法 入选经冠状动脉造影确诊为非急性心肌梗死的冠状动脉粥样硬化性心脏病患者为对照组；符合2012年全球统一心肌梗死定义的急性ST段抬高心肌梗死患者为实验组；对两组病例采集血液标本，采用qPCR 技术检测miRNA-1表达水平；同时对实验组进行ACC/AHA冠脉造影评分，比较两组间miRNA-1表达水平差异，并进一步分析实验组miRNA-1表达水平和ACC/AHA冠脉造影评分的相关性。结果入选42例病例，对照组21例，实验组21例。两组病例的micRNA-1相对表达量差异有统计学意义（P0.05）；并且实验组各病例的micRNA-1相对表达量和ACC/AHA冠脉评分存在显著相关性差异有统计学意义（相关系数R=0.6385，P0.05）。结论 循环miRNA-1和心肌梗死后心肌缺血程度存在显著相关性，miRNA-1有望成为新的检测心肌梗死和临床风险评估的分子标志物。 　　[关键词] miRNA-1；急性心肌梗死；心肌缺血 　　[中图分类号] R542.22 [文献标识码] A [文章编号] 1674-0742（2015）06（c）-0003-03 　　[Abstract] Objective To study the correlation between miRNA-1 and the degree of myocardial ischemia after acute myocardial infarction （AMI）. Methods The coronary atherosclerotic heart disease patients diagnosed with non-acute myocardial infarction by percutaneous transluminal coronary angioplasty （PTCA） were selected as the control group， and coronary atherosclerotic heart disease patients with AMI and acute ST segment elevation met the criteria of the 2012 Third Universal Definition of Myocardial Infarction were selected as the experimental group. Blood samples were obtained. The expression level of miRNA-1 was detected by real-time quantitative polymerase chain reaction（qPCR） detecting system. ACC/AHA score was evaluated in the experimental group. The expression of miRNA-1 was compared between the two groups. And the correlation between the expression of miRNA-1 and ACC/AHA score in the experimental group was further analyzed. Results 42 patients were selected and divided into the control group and the experimental group with 21 cases in each. There was statistically significant difference in the relative expression of miRNA-1（P=0.00020.01）. The relative expression of miRNA-1 was significantly associated with ACC/AHA score in patients in the experimental group（R=0.6385， P0.01）. Conclusion The expression of miRNA-1 after AMI is significantly associated with myocardial ischemia. miRNA-1 can act as a biomarker for the predic