Caveolin1和p42p44MAPK在哮喘大鼠气道平滑肌细胞增殖中作用.docVIP

Caveolin1和p42p44MAPK在哮喘大鼠气道平滑肌细胞增殖中作用 　　[摘要] 目的 观察哮喘大鼠肺组织中Caveolin-1和p-p42/p44MAPK含量的变化，探讨Caveolin-1和p42/p44MAPK在哮喘大鼠气道平滑肌细胞增殖中的作用。 方法 2009年4月～2010年6月间选择SPF级雄性SD大鼠24只，随机分为对照组（C组）、哮喘组（A组）和地塞米松组（D组），每组8只。以卵白蛋白（OVA）致敏和激发的方法复制大鼠慢性哮喘模型。观察肺组织病理超微结构变化，以图像分析软件测定支气管壁厚度（wat/pbm）和支气管平滑肌厚度（wam/pbm），免疫组化法检测气道平滑肌Caveolin-1、p-p42/p44MAPK蛋白的表达。 结果 A组大鼠wat/pbm、wam/pbm大于C组（P 0.01）；D组大鼠wat/pbm、wam/pbm小于A组（P 0.01），大于C组（P 0.01）；免疫组化法测定A组Caveolin-1表达量显著低于C组（P 0.01），D组表达高于A组（P 0.01），但低于C组（P 0.01）；A组p-p42/p44MAPK表达量显著高于C组（P 0.01），D组表达低于A组（P 0.01），但高于C组（P 0.05）；Caveolin-1表达与大鼠wam/pbm呈负相关（r = -0.894，P 0.01）；p-p42/p44MAPK表达与大鼠wam/pbm呈正相关（r = 0.805，P 0.01）；Caveolin-1与p-p42/p44MAPK表达呈负相关（r = -0.941，P 0.01）。 结论 Caveolin-1与p42/p44MAPK对哮喘大鼠气道重塑有一定影响，其相互作用对哮喘大鼠的气道平滑肌细胞增殖有调节作用。 　　[关键词] 哮喘；大鼠，气道重塑；Caveolin-1；p42/p44MAPK 　　[中图分类号] R562.2+5 [文献标识码] A [文章编号] 1673-9701（2013）25-0001-03 　　The role of caveolin-1 and p42/p44MAPK in the proliferation of airway smooth muscle cells in asthma 　　CHEN Huijun1 DAI Yuanrong2 　　1.Department of Respiratory Medicine， Jinhua Municipal Central Hospital of Zhejiang Province， Jinhua 321000， China； 2.Department of Respiratory Medicine， the Second Affiliated Hospital of Wenzhou Medical College， Wenzhou 325027， China 　　[Abstract] Objective To observe the changes of caveolin-1 and p-p42/p44MAPK in the lung tissue of asthmatic rats and to observe the effects of them in airway smooth muscle cells （ASMCs） proliferation. Methods Twenty-four healthy， male Sprague-Dawley rats from Apirl 2009 to June 2010 were randomly divided into three groups on average， including control group （group C）， asthmatic group （group A） and dexamethasone group（group D）. A rat chronic asthma model was exploited， in which rats were sensitized and provocated with ovalbumin. The histopathology and ultrastructure changes of pulmonary tissues were observed by light microscope. The total bronchial wall thickness （wat/pbm） and the bronchial smooth muscle thickness （wam/pbm） were measured with