GTR修复牙槽骨缺损beagle犬动物模型研究华楠.docVIP

GTR修复牙槽骨缺损beagle犬动物模型研究华楠 　　[摘要] 目的 为研究PLGA膜在beagle犬牙槽骨中的生物降解效果，建立一种符合牙槽骨缺损特点的动物模型。方法 该研究在2011年12月―2012年2月期间，以3只beagle犬为实验动物，年龄在14～16个月，平均体重（13.0±1.0）kg。拔除其双侧前磨牙。拔除牙后同时放入可吸收膜。在拔牙窝的左侧放置Bio-Guide胶原膜，右侧放置PLGA膜。 结果 3只实验犬均无死亡，饮食、活动正常。手术4周后再次进入牙槽骨，发现胶原膜和PLGA膜被完全吸收，生物降解。光学显微镜下显示在放置胶原膜和PLGA膜的拔牙窝上都形成了新的骨小梁。 结论 该研究采用的建模方法，成功构建了与临床即刻种植骨缺损相似，并可作为研究即刻种植骨界面骨性结合的实验动物模型。 　　[关键词] 牙槽；骨缺损；引导；组织再生；动物实验模型 　　[中图分类号] R780.1 [文献标识码] A [文章编号] 1674-0742（2015）09（b）-0048-02 　　[Abstract] Objective To study the biodegradation effect of PLGA film in the alveolar bone of beagle dogs， and establish a model of the animal model that was consistent with the characteristics of alveolar bone defect. Methods Between December 2011 and February 2012， 3 Beagle dogs aged from 14 to 16 months and with average weight of （13.0±1.0）kg， were selected as the experimental animals. We extracted their bilateral anterior molars， and then placed absorbable membrane in the extraction sockets with Bio-Guide collagen membrane in the left and PLGA membrane in the right. Results No mortality was found in all the dogs， and all of them showed normal diet and activity. Four weeks after extraction surgery， we observed the alveolar bone and found that the Bio-Guide collagen membrane and PLGA membrane were completely absorbed with good biodegradation effect. It was found under the optical microscope that new trabecular bone had developed at the extraction sockets where the absorbable membrane was placed. Conclusion Through the methods used in this study， we successfully establish the animal experimental model which is similar to immediate implantation in bone defect site and can also be applied in studying the synostosis of implant-bone interface. 　　[Key words] Alveolar； Bone defect； Guide； Tissue regeneration； Animal experimental model 　　引导骨再生技术（GBR）是针对骨的引导组织再生（GTR）技术，由GTR延伸而来[1]，GTR主要应用于牙齿[2]。目前，GBR主要运用的是胶原蛋白膜，然而它具有局限性，因此有必要研究合成聚酯纤维的可吸收生物膜。聚乳酸-羟基乙酸（PLGA）膜是一种可吸收生物膜，具有很好的生物降解性和生物相容性。而建立动物模型，研究PLGA膜在动物牙槽骨中的生物降解效果，才能进一步阐明PLG