P27和nm23H1在原发性乳腺癌组织中表达及其意义.docVIP

P27和nm23H1在原发性乳腺癌组织中表达及其意义 　　[摘要]目的 探讨P27和nm23-H1在乳腺癌中的表达及其与临床病理因素的相关性。方法 应用免疫组织化学S-P法, 检测我院1998~2008年间临床资料完整的女性原发性乳腺癌105例和50例乳腺纤维腺瘤标本(阴性对照组)中P27和nm23～H1的表达,分析2种基因之间的相关性及与转移的关系。结果 P27的阳性表达率在乳腺癌组明显低于对照组(P0.05)。在乳腺癌中,nm23-H1的阳性率在各临床分期组间比较有显著性差异(P0.01);P27和nm23-Hl的阳性率在各组织学分级组间比较有显著性差异(P0.O1);nm23-H1和P27的阳性表达率在有无淋巴结转移组之间比较有显著性差异(P0.05)。结论 联合检测乳腺癌组织中P27和nm23-H1的表达状况有助于判断预后。 　　[关键词]乳腺癌;P27;nm23-H1;预后;相关性 　　 [Abstract]Objective To investigate the expression of P27 and nm23 and their correlation with clinical pathological factors in primary breast carcinoma. Methods S-P method of Immunohistochemiistry was applied to detect the expressions of P27 and nm23-H1 in 100 cases of primary breast cancer and 50 cases of breast fibroadenoma. The correlations between the two genes and their relations with metastasis and prognosis were analyzed, all the cases admitted in our hospital from 1998 to 2008. Results The positive expression rate of P27 in breast cancer wasstatistically lower than that in fibroadenoma.while nm23-H1 was also lower than in fibroadenoma and showed no significance(P0.05). The positive expression rate of P27 and nm23-H1 were significantly associated with histological grading(P0.01). The positive expression rate of P27 and nm23-H1 were significantly associated between between groups with and without lymph node metastasis(P0.05).The expression of nm23-H1 was significantly associated with TNM stage(P0.01). Conclusion It could help to judge patients’ prognosis by jointly detecting the expression rate of P27 and nm23-H1 in primary breast carcinoma. 　　[Key words]Carcinoma of breast;P27;nm23-H1;Prognosis; Correlation 　　 　　乳腺癌是女性常见的恶性肿瘤之一,占我国妇女恶性肿瘤第二位,其发病率有逐年上升及年轻化的趋势。本文应用免疫组化方法对原发性乳腺癌手术标本的石蜡包埋组织中进行P27和nm23-H1表达的检测,是为了进一步探讨癌基因蛋白及细胞增殖活性,观察癌组织中的表达以及之间存在的关系,推测转移率,指导对预后的估计,同时为临床提供治疗依据。 　　 　　1. 资料与方法 　　 　　1.1 临床资料 　　随机选取本院1998年1月～2008年12月间进行乳腺癌根治术患者105例,均为女性,年龄27～64岁,平均54.6岁。术前均经病理检查确诊,并有详细的随访和预后资料,所有病例在手术时无远处转移。随访期为4～39个月,其中64例随访5年或5年以上,目前存活49例(死亡15例)。临床分期IIa期15例,II