iNOS及VEGF在兔角膜碱烧伤后角膜新生血管生成中作用研究.docVIP

iNOS及VEGF在兔角膜碱烧伤后角膜新生血管生成中作用研究 　　【摘要】目的研究诱导型一氧化氮合成酶（iNOS）和血管内皮生长因子（VEGF） 在碱烧伤后兔角膜新生血管（CNV）形成中的作用及其机制。方法用氢氧化钠滤纸片烧伤兔角膜建立新生血管动物模型，根据不同处理因素将实验兔分为三个实验组和一个对照组，每组12只。三个实验组结膜下注射不同剂量的三氧化二砷；对照组结膜下注射0.9%氯化钠。在裂隙灯下查看碱烧伤后角膜大体情况，计算角膜新生血管的面积。分别在兔角膜碱烧伤后7天、14天、28天，行兔角膜组织HE染色及免疫组化法检测iNOS和VEGF。 结果各实验组角膜新生血管面积均小于对照组 （P0.05或0.01）；实验组角膜比对照组更光滑透明；角膜iNOS和VEGF表达情况：各组碱烧伤后第7天开始增高，14天时达到峰值，随后逐渐降低，并且对照组的表达明显高于实验组。结论兔角膜碱烧伤后CNV形成与iNOS和VEGF密切相关；三氧化二砷对CNV形成具有一定抑制作用。 　　【关键词】一氧化氮合成酶；血管内皮生长因子；三氧化二砷；角膜新生血管 　　中图分类号：R774.1 文献标识码：ADOI：10.3969/j.issn2015.04.011 　　【Abstract】ObjectiveTo study effect and mechanism of inducible nitric oxide synthase （iNOS） and vascular endothelial growth factor（VEGF） in corneal neovascularization （CNV） after corneal alkali burn.MethodsAnimal models of neovascularization were established by burning rabbits corneas with sodium hydroxide filter papers.According to different kinds of treatments，experiment rabbits were divided into three experimental groups and one control group，with 12 rabbits in each group.The three experimental groups were given different doses of As2O3 by subconjunctival injection，while the control group were treated with 0.9% sodium chloride by subconjunctival injection.Corneas after alkali burn were observed under slit lamp，and areas of CNV were calculated.Rabbit corneal tissue HE staining and immunohistochemistry method were used to measure iNOS and VEGF 7，14 and 18 days after corneal alkali burn，respectively.Results Areas of CNV of all experimental groups were smaller than that of the control group（P0.05 or 0.01），and the corneas of the experimental groups were more smooth and transparent than that of the control group.Expressions of iNOS and VEGF in corneas of all groups began to increase on the 7th day and reached the peak on the 14th day after alkali burn，then decreased gradually.And the expressions of the control group were significantly higher than those of the experimental groups.ConclusionCNV formation after corneal alkali burn is closel