UVA1照射对人工皮肤分泌MMP1影响初步研究.docVIP

UVA1照射对人工皮肤分泌MMP1影响初步研究 　　[摘要]目的:探讨不同UVA1剂量照射对人工皮肤分泌MMP-1的影响,明确光致人工皮肤损伤的UVA1剂量。方法:通过ELISA法检测不同剂量的UVA1(0～90J/cm2)对人工皮肤表达MMP-1分泌的变化。结果:与0J/cm2相比,UVA1剂量为10J/cm2时MMP-1的分泌量无明显变化(P0.05),当UVA1剂量大于20J/cm2时人工皮肤分泌MMP-1的量逐渐增高(P0.05,P0.0001),UVA1剂量在小于50J/cm2以前呈剂量依赖性增高,至70J/cm2以后分泌量较前有所降低。结论:引起人工皮肤光损伤的初始UVA1照射剂量为20J/cm2。 　　[关键词]长波宽谱紫外线(UVA1);人工皮肤;基质金属蛋白酶-1(MMP-1) 　　[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2010)03-0348-03 　　 　　The preliminary study of the MMP-1 secretion in artificial skin in different dose of UVA1 　　SUN Su-jiao1,2,YAO Lu1,LUO Wen1,SU Shun-qin1,XU Ji-peng1,YNAG Zhi1,HE Li1 　　(1.Department of Dermatology, the First Affiliated Hospital of Kunming Medical College, Kunming 650032,Yunnan,China; 2.Department of Cosmetology, the Affiliated Hospital of Dali College, Dali671000,Yunnan, China) 　　 　　Abstract:Objective To explored the different dose ofUVA1 irradiation on artificial skin secretion of MMP-1, clearly the UVA1 dose oflight-induced damage in artificial skin. MethodsThe ELISA assay was used to measure the changes of secretion of MMP-1 after different doses of UVA1 exposure(0J/cm2～90J/cm2).ResultsCompared to dose of 0J/cm2, the secretion of MMP-1 of UVA1 dose of 10J/cm2 has no significant difference(P0.05). When the UVA1 doses greater than 20J/cm2, the secretion of MMP-1 in artificial skin was gradually increased (P0.05, P0.0001). The previous dose-dependent increase when UVA1 dose was less than 50J/cm2, but the secretion of MMP-1 was decreased when the dosage up to 70J/cm2.ConclusionThe results shows that the initial dose of the artificial skin photodamage caused by UVA1 radiation is 20J/cm2. 　　Key words:long-wave wide-spectrum ultraviolet (UVA1);artificial skin;matrix metalloproteinase -1 (MMP-1) 　　 　　近年来长波宽谱紫外线UVA1(340～400nm)对皮肤的光损伤越来越受到人们的重视,因为UVA1是紫外线中波长最长的一个波段,紫外线的波长越长对皮肤组织的穿透越深。UVA1的主要效应部位是皮肤真皮层,真皮层主要的细胞是成纤维细胞,一些实验证实真皮成纤维细胞产生的基质金属蛋白酶1(matrix metalloproteinase 1,MMP-1)与UVA引起的光损伤最为相关[1-2]。本实验应用人工皮肤,力求在一个模拟的更加接近正常人皮肤细胞环境的条件下,应用对皮肤穿透力更强的UVA1照射人工皮肤,观察不同U