HAC70多肽复合物疫苗对实验性肿瘤细胞抗肿瘤活性研究.docVIP

HAC70多肽复合物疫苗对实验性肿瘤细胞抗肿瘤活性研究 　　【中图分类号】R735.2【文献标识码】A【文章编号】1005-2720(2009)06-0137-03 　　【摘要】目的：观察从艾氏腹水瘤细胞中提取的热休克蛋白70-抗原肽复合物(HAC70)诱导的抗肿瘤免疫效应，探讨其抗肿瘤机制。方法：提取热休克后的小鼠艾氏腹水瘤细胞内热休克蛋白70(HSP70)经肝素-琼脂糖亲和层析纯化，制成热休克蛋白-肽复合物疫苗，以体内免疫法检测HAC70的抑瘤作用，3H-TdR掺入释放法检测脾细胞对肿瘤细胞的抗肿瘤活性。结果：经HAC70免疫的荷瘤小鼠皮下肿瘤结节平均重(1.26±0.4)g，较对照组(3.14±0.56)g明显减轻(P0.05)；当效靶比为200：1时，免疫组脾细胞杀伤活性为(49.25±11.25)%，显著高于对照组[(18.50±9.95)%，P0.01]及荷瘤组[(25.75±8.38)%，P0.01]。结论：经肝素-琼脂糖亲和层析纯化的HAC70可通过诱导机体产生特异性杀伤艾氏腹水瘤细胞的活性，明显抑制荷瘤小鼠艾氏腹水瘤的生长。 　　【关键词】艾氏腹水瘤细胞；HAC70-肽复合物疫苗；抗肿瘤活性 　　 　　The study of HAC70 effect of activities of anti-tumor on experiment tumor cell.Wang Yan，Liu Xin.(The first affiliated hospital of Liaoning medical college，Jinzhou 121001 China) 　　【Abstract】 Objective：To investigate the anti-tumor immuno-efficacy induced by HAC70 derived from Ehrlich ascites tumor cell and to study its mechanisms.Methods：After the heat-shock HSP70 from the Ehrlick ascites tumor cell was isilated and pureed by Heparin-Sepharose chromatography and got the HAC70 vaccine.The anti-tumor efficacy of splenocyte was determined by the 3H-TdR release assay.Results：The average tumor weight of the mice received HAC70 immunization was only(1.26±0.4)g，obvious difference(P0.05)，observed when contrasted with the control group(3.14±0.56)g.It was showed that specific cytotoxitic rate of splenocytes from the immunized mice to the Ehrlich ascites tumor cells was(49.75±8.93)%，significantly higher than from the Tumor-bearing(18.5±9.95)% and control group(25.75±8.38)%，P0.01.Conclusion：HAC70 pureed by Heparin-Sepharose chromatography have the activities of inflicting the Ehrlick ascites tumor cell and inhibit obviously growth of the Ehrlick ascites tumor. 　　【Key Words】 Ehrlich ascites tumor cell；HAC70 vaccine；Anti-tumor efficacy 　　 　　对热休克蛋白70-多肽复合物疫苗(HAC70)抗肿瘤作用的研究表明HAC70在诱导荷瘤机体产生抗肿瘤免疫效应的过程中，热休克蛋白(HSPs)的基因并没有突变，是伴随着HSPs分子上的抗原肽引起的免疫反应的特异性导致的抗肿瘤效应，比较重要的有HSP70、HSP90、GP96等。 　　本实验采用从艾氏腹水瘤细胞中提取的HSP70免疫BALB/c小鼠，制备热休克蛋白70-多肽复合物疫苗(HAC70)[1]，观察其抗肿瘤作用，并以3H-TdR释放法检测H