HIF1a VEGF在子宫内膜腺癌中表达与意义.docVIP

HIF1a VEGF在子宫内膜腺癌中表达与意义 　　【摘要】 目的 检测缺氧诱导因子-1a(hypoxia-inducible factor I alpha,HIF-1a),血管内皮生长因子(vascular endothelial growth factor,VEGF)在子宫内膜癌中的表达和微血管密度(microvessel density,MVD)的测定,探讨其与缺氧情况下子宫内膜癌血管生成的关系。方法 应用免疫组化S-P法检测46例子宫内膜腺癌中HIF-1a、VEGF的表达,并测定MVD值,用Spearman分析各指标间的相关性。结果1、子宫内膜癌中HIF-1a、VEGF的表达与对照组比较,差异有非常显著性意义(P0.01)。2、子宫内膜癌组织中HIF-1a、VEGF阳性表达与手术病理分期和肌层浸润程度无关,而与肿瘤病理分级呈正相关。MVD测定值与子宫内膜癌手术病理分期、病理分级和肌层浸润程度均有关。3、子宫内膜癌组织中HIF-1a阳性表达与VEGF阳性表达及MVD测定值之间存在显著正相关性。结论 1、子宫内膜癌组织中存在HIF-1、VEGF的过表达,HIF、VEGF与子宫内膜癌的发生发展有关。2、HIF-1通过调节VEGF的表达在子宫内膜癌血管生成中发挥着重要作用。 　　【关键词】子宫内膜癌缺氧诱导因子-1a血管内皮生长因子微血管密度 　　中国分类号:R737.33文献标识号:A 文章编号:1005-0515(2010)10-021-03 　　【Abstract】 Objectiveto detect the expression of hypoxia-inducible factor 1a(HIF-1a)and VEGF,P53 and MVD in endometrial carcinoma,and to investigate their association with angiogenesis of endometrial carcinoma under hypoxia. Methodsimmunohistochemical staining(s-p method)was used to detect HIF-1a and VEGF,P53and MVD expression in 46cases of endometrial adenocarcinoma,16cases of endometrial complex hyperplasia with atypical hyperplasia and 12cases of endometrial simple hyperplasia.Spearman coeffcient of correlation was used to test correlation between HIF-1a and VEGF,P53,MVD. Results 1、the expression of hypoxia-inducible factor 1a(HIF-1a)and VEGF in endometrial carcinoma is higherthan that in normal endometrial . 2、The expression of HIF-1alpha and VEGF in endometrial Carcinoma had no Relationship with surgical stages and muscular invasion.There were significant correlations between the expression of HIF-1 alpha and VEGF in endometrial careinoma and histological grading. Close positive relation was observed between microvessel density(MVD)level and surgical clinical stage,histological grading,muscular invasion of endometrial carcinoma. 3、HIF-1alpha expression was found significant association with VEGFand MVD expression. Conclusion1、There are overexpression of HIF-1a and VEGF in endometrial carcinoma,There were significant correlations bet