ICSI周期卵子成熟度对胚胎发育与临床结局影响.docVIP

ICSI周期卵子成熟度对胚胎发育与临床结局影响 　　【摘要】目的：研究卵胞浆内单精子注射（ICSI）周期中卵子成熟度对受精、胚胎发育以及临床结局的影响。方法：收集我院从2012年3月至2013年12月，新鲜周期中未成熟卵子（MⅠ或GV）占获卵数50%及以上的ICSI周期为A组（n=33）； 2012年9月至12月，新鲜周期中未成熟卵子小于获卵数的20%为ICSI周期为B组（n=138）。比较两组胚胎的受精率、正常受精率、多核率、优质胚胎率、临床妊娠率和种植率。结果：A组（n=33） 获卵261枚，成熟卵子118枚；其中受精率8051%，正常受精率7288%，多核率678%，优质胚胎率4737%，妊娠率3030%，种植率1930%；B组（n=138） 获卵1330枚，成熟卵子1146枚，其中受精率8997%，正常受精率8639%，多核率113%，优质胚胎率6164%，妊娠率5942%，种植率4088%。A组受精率、正常受精率、临床妊娠率、种植率均显著低于B组（P005），而多核率显著高于B组（P005）。结论：ICSI周期中，未成熟卵子比例过高可能影响胚胎的发育潜能及临床结局。 　　【关键词】卵子成熟度；卵胞浆内单精子注射；胚胎发育；临床妊娠率 　　The influence of oocyte maturation on embryo development and clinical outcomes in intracytoplasmicsperm injection cyclesJIANG Yiqun， WANG Shanshan， ZHANG Ningyuan， XU Zhipeng， SUN HaixiangΔ. Reproductive Medicine Center，The Affiliated Drum Tower Hospital of Nanjing University Medical School， Nanjing 210008， China 　　【Abstract】Objectives： To investigate the influence of oocyte maturation on fertilization， embryo development and clinical outcomes in intracytoplasmic sperm injection （ICSI） cycles. Methods： 33 patients with immature oocyte （MI oocytes or GV oocytes） accounted for more than half of the total in fresh cycle of ICSI cycles， from March 2012 to December 2013， were selected as group A. 138 patients， of whom the number of immature oocyte was less than 20% of the number of retrieved oocytes of ICSI cycles， from September 2012 to December 2012， were selected as group B. Fertilization rate， naomal fertilization rate， multinuclear rate， high quality embryos rate， clinical pregnancy rate and embryo implantation rate of the two groups were compared. Results： From group A， 261 oocytes were obtained； the number of mature oocytes was 118； the fertilization rate was 8051%； normal fertilization rate was 72.88%； multinuclear rate was 6.78%； high quality embryos rate was 47.37%； clinical pregnancy rate was 3030% and embryo implantation rate was 19.30%. From group B in， 1330 oocytes were obtained； the number of mature oocyte was 1146； the fertiliza