HPLC指纹法评价白芷饮片质量一致性.docVIP

HPLC指纹法评价白芷饮片质量一致性 　　 摘要：目的 对全国市售白芷饮片进行质量一致性的评价。方法 采用高效液相色谱法（HPLC）测定12份不同地区的市售饮片、1份硫磺熏蒸药材和1份自然晾干药材的指纹图谱;测定饮片中欧前胡素和异欧前胡素的含量。HPLC条件为：C18色谱柱（4.6 mm×250 mm，5 μm）;流动相为乙腈-水，梯度洗脱，流速1.0 mL/min;柱温35 ℃;检测波长选择210～800 nm范围的最大绘图。通过相似度计算方法进行分析比较。结果 建立了白芷饮片的HPLC指纹图谱分析方法，确定了12个共有特征峰。市售饮片的相似度为0.840～0.973;与硫磺熏蒸药材相比，指纹图谱的相似度为0.672～0.908;与自然晾干药材相比，指纹图谱的相似度为0.536～0.684。市售饮片中欧前胡素和异欧前胡素的含量范围分别为0.035%～0.140%和0.028%～0.069%。结论 不同地区的市售白芷饮片具有质量一致性，推测饮片均为硫磺熏蒸品。 　　 关键词：白芷;指纹图谱;饮片;欧前胡素;异欧前胡素;质量一致性 　　 DOI：10.3969/j.issn.1005-5304.2015.02.021 　　 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2015）02-0074-05 　　Evaluation of Quality Coherence of Angelicae Dahuricae Radix （Baizhi） Decoction Pieces Based on HPLC Fingerprint WU Xiao-yi1， CHAO Zhi-mao1， WANG Chun1，2， YU Li1，3， LIANG Yao-hua1， SUN Wen1， LIAO Yong-hong3 （1.Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China;2.The Institute of Basic Theory， China Academy of Chinese Medical Sciences， Beijing 100700， China;3.The School of Food， Beijing Technology and Business University， Beijing 100048， China） 　　 Abstract：Objective To evaluate the quality coherence of commercial Angelicae Dahuricae Radix （Baizhi） decoction pieces. Methods The samples of 12 batches of commercial decoction pieces， 1 batch of sulphur fumigated Baizhi， and 1 batch of naturally dried Baizhi were collected. HPLC method was used to determine the fingerprints and the contents of imperatorin and isoimperatorin by C18 column （4.6 mm×250 mm， 5 μm） with a gradient mobile phase of acetonitrile-water solution system at the flow rate of 1.0 mL/min. The column temperature was set at 35 ℃， and the max plot of detection wavelength was in 210-800 nm. Similarity calculation was used to analyze the data. Results The HPLC fingerprint analysis method was established with 12 common peaks. The similarities of 12 batches of commercial decoction pieces of Baizhi were 0.840-0.973. Their similarities compared with the sulphur fumigated Baizhi were