αB―crystallin在喉癌组织中表达及临床意义.docVIP

αB―crystallin在喉癌组织中表达及临床意义 　　[摘要] 目的 探讨αB-crystallin基因在喉癌组织中的表达及其与喉癌的关系。 方法 收集2008年3月～2014年10月南京医科大学第二附属医院及皖南医学院弋矶山医院手术切除的喉癌标本54例及13例癌旁正常黏膜标本，采用实时荧光定量PCR（Real-time PCR）检测8例喉癌组织及其癌旁正常组织中αB-crystallin基因的表达，免疫组化法检测54例癌组织及13例癌旁正常组织中αB-crystallin蛋白的表达，比较不同组织中αB-crystallin阳性表达率。 结果 喉癌组织中的αB-crystallin mRNA表达量及αB-crystallin蛋白阳性表达率均明显高于癌旁正常组织，差异均有高度统计学意义（P 0.05）。 结论 αB-crystallin基因的高表达与喉癌发生发展及转移有关，可能为喉癌患者的预后提供一个潜在的风险评估指标。 　　[关键词] αB-crystallin；实时荧光定量PCR；喉癌；免疫组化 　　[中图分类号] R739.63 [文献标识码] A [文章编号] 1673-7210（2016）10（a）-0009-04 　　Expression and significance of αB-crystallin in laryngeal squamous cell carcinoma 　　XIONG Lin1 MAO Yuan2 MA Jun3 ZHANG Dawei4▲ ZHU Jin5 CHEN Renjie4 　　1.Department of Pathology， the Second Affiliated Hospital of Nanjing Medical University， Jiangsu Province， Nanjing 210029， China； 2.Department of Otolaryngology-Head and Neck Surgery， Jiangsu Provincial Official Hospital， Jiangsu Province， Nanjing 210024， China； 3.Department of Otolaryngology-Head and Neck Surgery， Yiji Shan Hospital of Wannan Medical College， Anhui Province， Wuhu 241001， China； 4.Department of Otolaryngology-Head and Neck Surgery， the Second Affiliated Hospital of Nanjing Medical University， Jiangsu Province， Nanjing 210029， China； 5.Military Medical Research Institute of Nanjing Military Region， Jiangsu Province， Nanjing 210002， China 　　[Abstract] Objective To examine the expression of αB-crystallin in human laryngeal squamous cell carcinoma （LSCC） and investigate the relationship between its expression and LSCC. Methods A total of 54 cases of LSCC and 13 cases of adjacent normal tissues were collected from the Second Affiliated Hospital of Nanjing Medical University and Yiji Shan Hospital of Wannan Medical College from March 2008 to October 2014. Real-time quantitative polymerase chain reaction （8 LSCC tissues， 8 adjacent normal tissues） and immunohistochemistry （54 LSCC tissues and 13 adjacent normal tissues） were performed to detected the expression of αB-crystallin gene an