PTEN和PERK蛋白在皮肤鳞状细胞癌中表达和意义.docVIP

PTEN和PERK蛋白在皮肤鳞状细胞癌中表达和意义 　　[摘要]目的：探讨PTEN和P-ERK蛋白在皮肤鳞状细胞癌中的表达及意义。方法:采用免疫组化SP法观察34例皮肤鳞癌组织和15例正常皮肤组织石蜡标本中PTEN蛋白和P-ERK蛋白的表达情况。结果:皮肤鳞状细胞癌（SCC）组织中PTEN蛋白阳性表达率（41.18%）明显低于正常皮肤组织（100%）（P0.05）；P-ERK蛋白在SCC中的阳性表达率（76.47%）明显高于正常皮肤组织（20.00%）（P0.05）；SCC中低分化组的PTEN和P-ERK蛋白的阳性表达率分别低于和高于高分化组（P0.05）。SCC中PTEN和P-ERK蛋白表达之间呈明显的负相关（P0.01）。结论：PTEN蛋白的低表达或失表达，不能有效抑制Ras/Raf/MEK/ERK信号通路的异常激活，可能使细胞恶性转化和增殖。 　　[关键词]皮肤鳞状细胞癌（SCC）；PTEN蛋白；P-ERK蛋白；免疫组化；信号传导 　　[中图分类号]Q813[文献标识码]A[文章编号]1008-6455（2008）10-1483-03 　　 　　The expression and significance of PTEN and P-ERK protein in cutaneous squamous cell carcinoma 　　ZHOU Shu-wei,CHEN Min-jing 　　(Department of Plastic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052,Henan, China) 　　 　　Abstract:ObjectiveTo investigate the expression and significance of PTEN and P-ERK protein in cutaneous squamous cell carcinoma. MethodsExpression of PTEN and P-ERK protein was detected in 34 SCC and 15 normal skin specimens by SP immunohistochemical technique. ResultsThe positive rate of PTEN protein was significantly lower in SCC(41.18%) than that in normal skin(100%)(P0.05); The positive rate of P-ERK was significantly higher in SCC(76.47%) than that in normal skin (20.00% )(P0.05); The positive rate of PTEN and P-ERK protein in the low differentiation group were lower and higher than them in the high differentiation group(P0.05), A significant negative relationship was observed between the expression of PTEN and P-ERK protein in SCC(P0.01).ConclusionsThe decrease or deletion of PTEN protein expression may not be able to effectively inhibit the over-activation of Ras/Raf /MEK/ERK signaling pathway, which will result in the m alignant transformation and proliferation. 　　Key words: cutaneous squamous cell carcinoma; PTEN protein; P-ERK protein; Immunohistochemistry;signal transduction 　　 　　皮肤鳞状细胞癌(cutaneous squamous cell carcinoma, SCC)的发生发展与抑癌基因的失活、突变及某些细胞信号的传导异常密切相关。PTEN是迄今发现的第一个具有双特异性磷酸酶活性的抑癌基因[1]。PTEN主要通过脱磷酸化作用调控细胞内信号传导通路，从而调节细胞的生长、