HWTXI对大鼠脑缺血再灌注后脑线粒体中自由基损伤保护作用.docVIP

HWTXI对大鼠脑缺血再灌注后脑线粒体中自由基损伤保护作用 　　摘要：目的：研究虎纹毒素(HWTX?I)对大鼠脑缺血再灌注后脑线粒体MDA含量、SOD和GSH?Px活性的影响，探讨HWTX?I的脑保护作用机制。方法：对脑缺血损伤的SD大鼠在缺血的同时蛛网膜下腔注射HWTX?I，再灌注24 h后测定脑线粒体MDA含量、SOD和GSH?Px活性。结果：脑缺血再灌注后大鼠脑线粒体MDA含量明显增加，SOD、GSH?Px活性明显降低（P＜0.05）；与生理盐水对照组相比，HWTX?I（1ug/kg）治疗组上述指标均有明显改善（P＜0.05）。结论：HWTX?I对缺血再灌注后脑线粒体自由基损伤有一定的保护作用。? 　　关键词：大鼠；HWTX?I；脑缺血再灌注；线粒体；自由基? 　　中图分类号：G804.7文献标识码：A文章编号：1007-3612(2008)01-0044-03? 　　On the Protective Function of HWTX?I From the Damage of Rats Cerebral Ischemia to ?Hindbrains Mitochondrial Free Radical after Reperfusion? 　　ZHOU Hong?yan?1，MAO Hai?feng?2，CHEN Jia?qin?2? 　　(1. Department of Physical Education of University of Arts and Sciences, Changde 415000, Hunan China;?2. Academy of Physical Education, Hunan Normal University, Changsha 410081, Hunan China)? 　　Abstract:The thesis was aimed at studying the influence of HWTX?I on rats hindbrain content ofmitochondrial MDA, the activities of SOD and GSH?Px after the cerebral ischemia and reperfusion. It explored HWTX?Is protective mechanism on encephalon. The methods of the research were to inject HWTX?I on subarachnoid cavity while the SD rat which bears the damage of cerebral ischemia was still in the stage of ischemia, and to determine the content of hindbrain mitochondrial MDA and the activities of SOD and GSH?PX after it has been reperfused for twenty?four hours. It was shown that content of hindbrain mitochondrial MDA will be remarkably increased while the activities of SOD and GSH?Px be decreased in the same time. Compared with the salt water group, the index of HWTX?I therapeutic group has obviously been improved. It is concluded that HWTX?I has protective function on the damage of cerebral ischemia to the free radical of hindbrain mitochondrial after reperfusion.? 　　Key words: rat;Huwenatoxin?I;cerebral ischemia?reperfusion;mitochondria;free radical? 　　? 　　竞技体育比赛充满激烈的对抗性和竞争性，在各种体育训练和比赛中，遭受运动性创伤甚至引起伤残是运动员经常要面对的事实。而颅脑损伤是运动创伤中较为常见的创伤性疾病，严重的颅脑损伤会造成运动员中枢神经系统的缺血、缺氧，引起神经元凋亡和坏死，其致残率和死亡率均极高。本研究试图通过缺血性