Toll样受体3在大鼠急性心肌梗死中作用机制.docVIP

Toll样受体3在大鼠急性心肌梗死中作用机制 　　[摘要] 目的 研究Toll样受体3（TLR3）在大鼠心肌梗死中的表达及作用机制。 方法 制备TLR3基因敲除大鼠及野生型大鼠，采用球囊结扎冠脉方法制作心肌梗死动物模型。随机分为野生型假手术组（n=15）、野生型心肌梗死组（n=15）、（TLR3-/-）假手术组（n=15）、（TLR3-/-）心肌梗死组（n=15）。检测心肌组织肌酸激酶同工酶（CK-MB）、肌钙蛋白cTnI含量；通过Western blot法检测Toll样受体3、NF-κB表达。 结果 与野生型心肌梗死组大鼠比较，（TLR3-/-）心肌梗死?M反映心肌损伤的CK-MB、cTnI减低；NF-κB表达降低；各组间差异均有统计学意义（P0.05）。 结论 TLR3基因缺失在急性心肌梗死中具有心脏保护作用，其保护机制与下调NF-κB表达，减轻心肌梗死炎症反应有关。 　　[关键词] TOLL样受体3；急性心肌梗死；核因子-κB；基因敲除 　　[中图分类号] R541；R392 [文献标识码] A [文章编号] 1673-9701（2018）01-0036-03 　　Mechanism of Toll-like receptor 3 in acute myocardial infarction in rats 　　WANG Yuanyuan1 CAO Jian2 DENG Li1 　　1.Department of Cardiology，the Third Hospital of Nanchang， Nanchang 330009，China； 2.Department of Anesthesiology，the Second Affiliated Hospital of Nanchang University，Nanchang， 330006，China 　　[Abstract] Objective To study the expression of Toll-like receptor 3（TLR3） in myocardial infarction of rats and its mechanism. Methods TLR3 gene knockout rats and wild-type rats were prepared. Animal models of myocardial infarction were created by balloon ligation of coronary arteries. The rats were randomly divided into wild-type sham surgery group （n=15）， wild-type myocardial infarction group（n=15），（TLR3-/-）sham surgery group（n=15）， and（TLR3-/-）myocardial infarction group（n=15）. The creatine kinase（CK-MB）and troponin cTnI contents in myocardial tissues were measured. Toll-like receptor 3 and NF-κB expressions were detected by Western blot method. Results Compared with the rats in the wild-type myocardial infarction group， the levels of CK-MB and cTnI which reflected myocardial injury in the（TLR3-/-） myocardial infarction group were decreased； NF-κB expression was decreased； the differences between groups were statistically significant（P0.05）. Conclusion TLR3 gene deletion has cardioprotective effects in acute myocardial infarction. Its protective mechanism is related to the down-regulation of NF-κB expression and alleviation of inflammatory response induced by myocardial infa