不同临床分期对原发性肝癌患者血清VEGFTNF―α表达影响.docVIP

不同临床分期对原发性肝癌患者血清VEGF、TNF―α表达的影响 　　【摘要】 目的：探讨原发性肝癌（PHC）患者血管内皮生长因子（VEGF）活性和肿瘤坏死因子-α（TNF-α）水平的变化及临床意义。方法：选择63例PHC患者，采用2001年中国抗癌协会肝癌专业委员会制定的PHC诊断及分期标准分为Ⅱ、Ⅲ、Ⅳ期，另选取30例健康体检者作为对照组，检测所有受试者VEGF、TNF-α表达的情况。结果：不同分期PHC患者血清中VEGF、TNF-α含量均显著高于对照组（P0.01）； Ⅱ、Ⅲ、Ⅳ期PHC患者VEGF、TNF-α含量逐渐增高；Ⅱ期VEGF、TNF-α含量明显低于Ⅲ期 （P0.05）；Ⅳ期VEGF、TNF-α水平显著高于Ⅱ期 （P0.01）。结论：VEGF、TNF-α的表达水平可以作为判断PHC发生发展、分化程度及转移的检测指标。 　　【关键词】 原发性肝癌； 肝癌分期； 血管内皮因子； 肿瘤坏死因子-α 　　【Abstract】 Objective：To investigate the levels change and clinical significance of vascular endothelial growth factor（VEGF） activity and tumor necrosis factor alpha（TNF-α） in primary liver cancer（PHC）.Method：A total of 63 patients with PHC were chosen，using Chinese anti-cancer association professional committee of liver cancer in 2001 formulated the PHC diagnosis and staging of the standard is divided into phase Ⅱ，phase Ⅲ，Ⅳ period，and the other 30 cases of healthy physical examination were selected as blank control group， VEGF and TNF-α of all objects were compared.Result：Different stages PHC patients serum VEGF and TNF-αlevels were significantly higher than that of blank control group（P0.01）.Phase Ⅱ，Ⅲ，Ⅳ period PHC patients with VEGF，TNF-αcontent increased gradually.Phase Ⅱ VEGF，TNF-αwere significantly lower than those of phase Ⅲ（P0.05）.Phase Ⅳ VEGF and TNF-α were significantly higher than those of phase Ⅱ（P0.01）.Conclusion：The expression of VEGF and TNF-α can be used as a judgment of PHC detection index of the development，differentiation and metastasis. 　　【Key words】 Primary liver cancer； Cancer of liver stages； Vascular endothelial factor； Tumor necrosis factor-α 　　First-author’s address：Shenzhen City Baoan District Songgang People’s Hospital，Shenzhen 518105，China 　　doi：10.3969/j.issn.1674-4985.2016.21.031 　　原发性肝癌（primary hepatic cancer，PHC）是指发生在肝细胞或肝内胆管细胞的肿瘤[1]。新生血管的形成对于肝癌细胞的恶性转化及远处扩散至关重要[2]，血管内皮生长因子（vascular endothelial growth factor，VEGF） 是目前已知的、调节血管形成的关键因子，特异性很高[3]。肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）是重要的前炎性细胞因子，具有抗肿瘤作用[4-5]。本研究通过观察PHC患者血清VEGF及TN