不同蒸制时间对人参活性成分及其药代动力学行为与抗炎作用影响研究.docVIP

不同蒸制时间对人参活性成分及其药代动力学行为与抗炎作用的影响研究 　　[摘要]选用优质人参分别高压蒸制0，2，4，8 h后制备人参提取物，并利用HPLC研究人参蒸制前后的化学成分发生的变化；采用UFLCMS/MS多反应监测（MRM） 定量分析，以地高辛为内标物在负离子模式条件下，研究灌服不同蒸制时间的人参提取物的小鼠体内人参皂苷类成分的药代动力学行为差异；以及通过给灌胃人参提取物1周后的小鼠腹腔注射脂多糖（LPS），建立炎症模型，运用酶联免疫法检测炎症模型小鼠血浆中的肿瘤坏死因子α（TNFα）和白细胞介素1β （IL1β）的含量，探讨不同蒸制时间对人参发挥抗炎作用的影响。经测定灌服蒸制8 h的人参提取物可明显降低炎症模型小鼠血浆中的TNFα和IL1β含量。研究结果表明人参经蒸制后化学成分发生变化，入血成分相应改变，人参经蒸制8 h后有助于起到抗炎效果，为进一步揭示人参发挥抗炎作用的物质基础和量效关系提供实验依据。 　　[关键词]人参；蒸制；人参皂苷；TNFα；IL1β 　　[Abstract]HPLC analysis was performed to study the changes in chemical composition of ginseng extracts prepared from high quality ginseng with 0， 2， 4， 8 h of steamed times An UFLCMS/MS multiplereaction monitoring （MRM） quantitative analysis was made to investigate the pharmacokinetic behavior differences of ginsenosides in mice ig administered of ginseng extracts with different steamed times in the negative ion mode， with Digoxin as the internal standard substance The mice were injected with LPS to establish inflammation model after ig administration of ginseng for a week and the contents of tumor necrosis factorα （TNFα） and interleukin1β （IL1β） in mice plasma were detected by ELISA， in order to study on antiinflammatory effects of ginseng with different steamed times It was determined that levels of TNFα and IL1β were significantly decreased in inflammation model group ig administered of ginseng extracts with 8h of steamed time The results showed that the chemical components in ginseng changed after steaming and the components into the blood changed， correspondingly Ginseng with steamed 8 h contributes to antiinflammatory effects These results provided an experimental basis for revealing the active substance basis and doseeffect relationship of ginseng on antiinflammatory effect 　　[Key words]ginseng； steaming； ginsenoside； TNFα； IL1β 　　人参Panax ginseng CAMeyer为五加科多年生草本植物人参的根，素有“百草之王”的美称，是我国传统名贵中药，具有大补元气、固脱、生津、安神和益智的作用。人参经洗净烘干后为生晒参，经烘干蒸制加工后的为红参。传统中医学认为“红参偏热、生晒参偏凉”。现代药理学研究表明人参具有免疫调节、抗癌、抗脑缺血、治疗糖尿病、预防动脉粥样硬化和抗炎等药理学活性，经蒸制后抗癌活性会增加[