丁酸钠诱导HepG2细胞凋亡机制研究.docVIP

丁酸钠诱导HepG2细胞凋亡机制的研究 　　【摘要】目的：探讨丁酸钠对HepG2细胞凋亡的影响及其作用机制。 方法：采用MTT法观察不同浓度丁酸钠对HepG2细胞增殖的抑制作用；应用流式细胞仪检测丁酸钠作用于HepG2细胞后的凋亡情况；TRAP-ELISA法检测细胞端粒酶活性的变化；RT-PCR技术分析人端粒酶逆转录酶（human telomerase reverse transcriptase， hTERT）mRNA的表达水平。 结果：丁酸钠作用HepG2细胞能明显抑制细胞增殖，其作用具有时间和剂量依赖性。丁酸钠处理48h的HepG2细胞凋亡率明显提高（P0.01），S期细胞比例显著下降（P0.05），G0/G1期细胞比例显著升高（P0.05）。经丁酸钠处理的实验组端粒酶活性为（1.16±0.12），未经丁酸钠处理的对照组端粒酶活性为（3.58±0.33），二者之间差异有显著性（P0.05）。丁酸钠处理的实验组hTERTmRNA水平（0.177±0.063），与对照组（0.589±0.152）比较差异有统计学意义（P0.01）。 结论：丁酸钠具有强烈诱导HepG2细胞凋亡的作用，其发生机制与丁酸钠下调hTERTmRNA表达水平直接相关。 　　【关键词】丁酸钠；HepG2细胞；凋亡 　　文章编号：1009-5519（2007）22-3322-02 中图分类号：R36 文献标识码：A 　　Study on the mechanism of apoptosis in HepG2 cell line induced by sodium butyrate 　　HU Xiao-qian 　　（Department of General Surgery，The Second Peoples Hospital of Macheng，Hubei438307，China） 　　【Abstract】Objective：To explore the effect on cell apoptosis of HepG2 cell line and its mechanism.Methods：The proliferative activity of HepG2 cell line was observed by MTT assay. Flow cytometry was used to assess cell apoptosis after being treated by sodium butyrate. And TRAP-ELISA was applied to analyze telomerase activity of HepG2 cell. In addition， the expression of telomerase hTERT mRNA was confirmed by RT-PCR technology.Results：A time and dose dependent inhibition was remarkably confirmed in HepG2 cell line. As compared with control group，the apoptosis rate of HepG2 cell line in research group was increased obviously after being treated with sodium butyrate for 48 hours（P0.01）. The percentage of S phase decreased significantly（P0.05），while the percentage of G0/G1 phase was increased markedly（P0.05）. The activity of telomerase（1.16±0.12） in research group was significantly slower than that（3.58±0.33） in control group（P0.05）. Furthermore，the expression level of hTERT mRNA（0.177±0.063） in research group was significantly slower than that（0.589±0.152） in control group（P0.01）. Conclusion：Sodium butyrate can greatly induce apoptosis ofHepG2 cell line， and the decline of expression l