丁香酚乳液和醇溶液对鲤鱼麻醉作用对比研究.docVIP

丁香酚乳液和醇溶液对鲤鱼麻醉作用的对比研究 　　摘要:以吐温80、司盘80、无水乙醇和水为原料制成丁香酚乳液,对鲤鱼进行麻醉,对比测定丁香酚乳液和丁香酚醇溶液对鲤鱼的麻醉开始时间、麻醉持续时间和LD50。结果表明:在相同浓度下,丁香酚乳液对鲤鱼的麻醉开始时间比丁香酚醇溶液慢,作用较平缓,但麻醉持续时间长5倍。丁香酚乳液的半数致死量比丁香酚醇溶液大2.15倍。因此,丁香酚乳液对鲤鱼的麻醉作用较缓和,麻醉维持时间长,安全性更高,更适合用于生产实践。 　　关键词:丁香酚;乳化;麻醉 　　中图分类号:S965.116文献标识码:A DOI编码:10.3969/j.issn.1006-6500.2009.03.001 　　 　　Contrast Study of Anesthetic Effect of Emulsified Eugenol and Alcoholic Eugenol on Cyprinus carpio Linnaeus 　　LV Shi-ming1,CAI Hong-neng2,TAN Ai-juan2,ZHANG Fu-lin1,YIN Ze-dong1 　　(1.College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China;2. College of Life Science,Guizhou University,Guiyang,Guizhou 550025,China) 　　Abstract:To test the anesthetic effect of emulsified eugenol to Cyprinus carpio Linnaeus, eugenol was emulsified with Tween 80, Span 80, ethanol and water. Then the start time, time length of anesthesia and LD50 of emulsified eugenol to Cyprinus carpio Linnaeus was determined contrastively with alcoholic eugenol. Results showed that the start time of anesthesia of emulsified eugenol was longer than these of alcoholic eugenol under the same concentration of eugenol. The time length and LD50 of anesthesia of emulsified eugenol were five times longer and 2.15 times large respectively than that of alcoholic eugenol. So, the anesthetic effect of emulsified eugenol on Cyprinus carpio Linnaeus was slower, gentler, longer and safer than alcoholic eugenol. It was more proper to be applied for fish farming than alcoholic eugenol as a fish anesthetic agent. 　　Key words:eugenol;emulsion;anesthesia 　　 　　丁香酚(Eugenol)是一种油状的有香味的化合物,是很多中草药如丁香、肉桂、肉豆蔻中的有效化学成分[1]。作为临床常用药,丁香酚具有广泛的药理活性,尤其是在抗菌消炎、杀虫驱虫、抗氧化和抗衰老等方面的生理活性具有较大的利用价值[2,3]。但由于其挥发性强、与水不相混溶的特性,大大限制了其作为水产品麻醉剂的应用。为改善丁香酚对鱼类的麻醉效果,对丁香酚进行乳化,并就其麻醉效果与丁香酚醇溶液[4]进行了比较研究,以期为丁香酚在生产中的应用提供试验依据和理论参考。 　　 　　1材料和方法 　　 　　1.1材 料 　　药品:丁香酚、吐温80、司盘80、无水乙醇、自来水;动物:体长15～20 cm鲤鱼若干;器具:15 L塑料盆若干,增氧泵1个;水温:供试水温为10～15 ℃。 　　1.2方 法 　　1.2.1丁香酚乳液的配制按吐温80∶司盘80∶无水乙醇∶丁香酚 = 4∶2∶1∶2的比例配制乳液,首先将吐温80与司盘80混合,然后再加入乙醇和丁香酚,最后缓慢