七叶亭对大鼠血管平滑肌细胞凋亡调节作用及分子机制研究.docVIP

七叶亭对大鼠血管平滑肌细胞凋亡的调节作用及分子机制研究 　　[摘要] 目的 研究七叶亭对大鼠血管平滑肌细胞凋亡的调节作用及分子机制。方法 离体培养大鼠血管平滑肌细胞，分为高剂量组、中剂量组、低剂量组和对照组。采用MTT法检测细胞的凋亡情况，采用荧光定量PCR检测细胞中Bcl-2、Bax和caspases-9的mRNA含量。 结果 ①细胞凋亡：七叶亭处理后MTT值均低于对照组，且药物剂量越高、MTT值越低；②基因mRNA含量：七叶亭处理后，Bcl-2的mRNA含量低于对照组，Bax和caspases-9的mRNA含量高于对照组；且药物剂量越高，Bcl-2的mRNA含量越低，Bax和caspases-9的mRNA含量越高。结论 七叶亭处理能够诱导大鼠血管平滑肌细胞发生凋亡，且抑制Bcl-2表达和促进Bax、caspases-9表达可能是药物发挥作用的分子机制。 　　[关键词] 七叶亭；血管平滑肌细胞；凋亡；血管再狭窄 　　[中图分类号] R719.8 [文献标识码] A [文章编号] 1674-0742（2015）09（a）-0004-02 　　Study on the Regulation Effect and Molecular Mechanisms of Esculetin on Rat Vascular Smooth Muscle Cell Apoptosis 　　LI Li 　　Department of Cardiovascular Medicine， Huadu District， Guangzhou City Peoples Hospital， Guangzhou， Guangdong Province， 510800 China 　　[Abstract] Objective To study the regulation effect and molecular mechanisms of esculetin on rat vascular smooth muscle cell apoptosis. Methods Rats vascular smooth muscle cells were cultured and divided into high-dose group， middle-dose group， low-dose group and control group. Cell apoptosis was detected by MTT assay. Bcl-2， Bax and caspases-9 mRNA contents were detected by quantitative PCR. Results ①Cell apoptosis： after esculetin treatment， MTT values were lower than control group and the higher the dose， the lower the MTT value； ②mRNA content： after esculetin treatment， Bcl-2s mRNA levels were lower than control group， Bax and caspases-9 mRNA levels were higher than control group； the higher the dose， the lower the mRNA levels of Bcl-2 and the higher the mRNA levels of Bax and caspases-9. Conclusion Esculetin treatment can induce the apoptosis of rat vascular smooth muscle cells and inhibit the Bcl-2 expression and promote Bax and caspases-9 expression， which may play a role in the molecular mechanisms of drugs. 　　[Key words] Esculetin； Vascular smooth muscle cells； Apoptosis； Vascular restenosis 　　血管再狭窄是冠心病患者冠状动脉腔内成形术后常见的并发症，会严重影响疾病的预后。近年来的研究认为，血管平滑肌细胞增殖是造成血管再狭窄的重要途径，诱导血管平滑肌细胞凋亡也成为了预防血管再狭窄的重要靶点[1]。七叶亭是一类天然香豆素类化合物，具有诱导肿瘤细胞凋亡、保护心肌细