乙型肝炎病毒DNA与乙肝两对半模式关系临床分析.docVIP

乙型肝炎病毒DNA与“乙肝两对半”模式关系的临床分析 　　[摘要] 目的 探讨乙型肝炎病毒DNA与“乙肝两对半”模式关系。方法 整群选取2013年9月―2014年9月于该院确诊的236例乙型病毒性肝炎患者，采用酶联免疫吸附测定（ELISA）检测患者“乙肝两对半”血清指标，包括HBsAg，HBsAb，HBeAg，HBeAb及HBcAb；采用荧光定量聚合酶链反应（FQ-PCR）检测定量患者HBV-DNA，对“乙肝两对半”与HBV-DNA进行相关性分析。结果 不同“乙肝两对半”模式的患者HBV-DNA阳性率不同，差异有统计学意义（χ2=34.25，P0.05）。不同“乙肝两对半”模式的患者HBV-DNA含量不同，乙肝两对半中HBsAg（+）， HBeAg（+）和HBcAb（+）模式患者的HBV-DNA阳性率及HBV-DNA含量最高，为95.31%及（6.47±1.24）。HBsAg（+）模式的患者HBV-DNA阳性率均高于HBeAg（-）模式，差异有统计学意义（P0.05）。“乙肝两对半”模式与HBV-DNA阳性率相关性较为显著（χ2=35.63，r=0.30，P0.05）。 结论 诊断乙型病毒性肝炎同时采用“乙肝两对半”模式与HBV-DNA时，检测结果具有较高诊断价值，可作为其诊断与疗效评估的可靠指标。 　　[关键词] 乙型肝炎；病毒DNA；乙肝两对半；模式关系 　　[中图分类号] R4 [文献标识码] A [文章编号] 1674-0742（2015）09（b）-0105-02 　　[Abstract] Objective To discuss the relationship between detection of HBV-DNA and hepatitis B serum five markers. Methods From September 2013 and September 2014， 236 HBV patients confirmed in this hospital underwent detection of hepatitis B serum five markers （HBsAg， HBsAb， HBeAg， HBeAb， HBcAb） using enzyme linked immunosorbent assay（ELISA）， and detection of HBV-DNA using fluorescent quantitative polymerase chain reaction（FQ-PCR）. We analyzed the correlation of the two detection modes. Results Patients with different modes of hepatitis b two fifty-fifty showed varying positive rate of HBV-NDA， and the difference was statistically significant（χ2=34.25， P0.05）. The positive rate and levels were highest in patients with HBsAg（+）， HBeAg（+） and HBcAb（+）， which were 95.31% and （6.47±1.24） copies/mL， respectively. The HBV-NDA positive rate was higher in patients with HBsAg（+）than in those with HBeAg（-）with statistically significant difference. Overall， there was significant correlation between hepatitis b two fifty-fifty modes and HBV-NDA positive rate，χ2=35.63， r=0.30， P0.05. Conclusion Detection of HBV-DNA and hepatitis B serum five markers has high value in diagnosis of HBV and can be used as a reliable indicator for diagnosis and evaluation of efficacy. 　　[Key words] Hepatitis B； Virus DNA； Hepatitis b two fifty-fifty； Relati